Artificial Synthetic Test Soil ISO/TS 15883-5 – for Washer-Disinfector Cleaning Efficacy Testing BZ489
Product Code: BZ489
Manufacturer: Biochemazone
Available Pack Sizes: Dried Powder: 5 g, 10 g, 20 g, 50 g – for making 50 mL, 100 mL, 200 mL, 500 mL · Premixed Ready to Use Formulation: 50 mL, 100 mL, 200 mL, 500 mL
Custom Bulk Quantities Available
Product Overview
Biochemazone Artificial Synthetic Test Soil BZ489 is formulated to demonstrate the cleaning efficacy of washer-disinfectors and their accessories used for reusable medical devices, in line with ISO/TS 15883-5. It is a synthetic blend that simulates the proteins, lipids and body fluids which build up on medical, dental, laboratory, pharmaceutical and veterinary equipment. It is non-toxic, free from blood products and bright red, so that soil left after the cycle is easy to see.
The soil is applied to instruments or test carriers as a paste, left to dry for about 30 minutes and put through the normal cycle; red residue afterwards shows where cleaning was incomplete. BZ489 is used for cleaning efficacy validation, for routine and quarterly checks, for testing after maintenance, and for finding the weak points of a washer-disinfector that needs adjustment.
BZ489 can be ordered as Dried Powder or as a Premixed Ready to Use Formulation – the powder in 5 g, 10 g, 20 g and 50 g packs for making 50 mL, 100 mL, 200 mL and 500 mL, and the premixed formulation in 50 mL, 100 mL, 200 mL and 500 mL packs. The dried powder has a 2-year shelf life and can be reconstituted easily. Unopened, it has a 2-year shelf life at 2–30 °C and is shipped at room temperature. For the soil that is spread in the chamber see Washer Disinfector Soil Test (BZ487); for a soil with protein, hemoglobin and carbohydrate markers see Artificial Test Soil ASTM F3208-20 (BZ477).
Two Versions to Select
- Dried Powder: Soil powder that is mixed with water and red indicator ink to a uniform paste before the test; the ink is bought separately.
- Premixed Ready to Use Formulation: Supplied mixed and ready to apply, with no weighing or mixing.
Select the version first and then the pack size. The Dried Powder has a 2-year shelf life and can be reconstituted easily: 5 grams is for making 50 mL, 10 grams is for making 100 mL, 20 grams is for making 200 mL and 50 grams is for making 500 mL. The Premixed Ready to Use Formulation is supplied in 50 mL, 100 mL, 200 mL and 500 mL packs.
How to Use
- Prepare the soil paste: mix the Dried Powder with water and red indicator ink, or use the Premixed Ready to Use Formulation as supplied.
- Apply: spread the paste on the instrument surfaces or test carriers, covering every area to be tested.
- Dry: leave the soil to dry for about 30 minutes, so that it bonds to the surface.
- Run the cycle: load the items and start the standard cleaning cycle.
- Inspect: check the items for red residue, which marks incomplete cleaning.
Key Features & Benefits
✅ ISO/TS 15883-5 – For demonstrating the cleaning efficacy of washer-disinfectors
✅ Simulates Complex Soiling – Proteins, lipids and body fluids found on reusable devices
✅ Bright Red Indicator Colour – Incomplete cleaning is easy to see
✅ Non-Toxic, No Blood Products – Safe to handle
✅ Two Versions to Select – Dried Powder (5 g to 50 g, for making 50 mL to 500 mL) or Premixed Ready to Use Formulation
✅ 2-Year Shelf Life – Unopened at 2–30 °C; shipped at room temperature
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🚿 Cleaning Efficacy Validation
• Washer-disinfectors for reusable medical devices
• Washer accessories and load carriers
• ISO/TS 15883-5 testing
📅 Routine Checks
• Daily, quarterly or as-needed testing
• Monitoring of cleaning processes
• Infection prevention and control programmes
🔧 Troubleshooting & Maintenance
• Weak spots in the wash chamber
• Testing after maintenance
• End-of-line testing
🏥 Sectors
• Medical and dental
• Laboratory and pharmaceutical
• Veterinary
Typical Properties
- Composition: Synthetic blend of non-toxic soiling agents that simulate proteins, lipids and body fluids; no blood products
- Colour: Bright red
- Standard: ISO/TS 15883-5
- Versions: Dried Powder (mix with water and red indicator ink); Premixed Ready to Use Formulation
- Dried Powder packs: 5 g, 10 g, 20 g, 50 g – for making 50 mL, 100 mL, 200 mL, 500 mL
- Premixed Ready to Use Formulation packs: 50 mL, 100 mL, 200 mL, 500 mL
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, in the tightly closed container, protected from light and moisture
- Transportation: At room temperature
- Shelf Life: 2 years (unopened)
- Versions: Dried Powder and Premixed Ready to Use Formulation – the 2-year shelf life applies to both, unopened
- Dried Powder: 2-year shelf life and easy to reconstitute – 5 grams is for making 50 mL, 10 grams for 100 mL, 20 grams for 200 mL and 50 grams for 500 mL
- After Reconstitution or Opening: Close tightly after use and keep dry; prepare only the amount needed for the test
Customization Options
Customization available on request:
- Soil adapted to simulate a particular type of clinical soil
- Pack sizes for your testing schedule
- Custom pack sizes and bulk quantities
Frequently Asked Questions
How often should BZ489 be used?
We recommend quarterly testing, in line with ISO/TS 15883-5 and HTM 01-05 practice. More frequent testing may be needed, depending on your risk assessment and the number of devices processed.
Can it be used in all washer-disinfectors?
Yes. It is suitable for the washer-disinfectors used in hospitals, dental clinics, laboratories and reprocessing units.
Is it safe to handle?
Yes. BZ489 is non-toxic and contains no blood products.
How does BZ489 differ from BZ487?
Both are bright red, blood-free soils for washer-disinfectors. BZ489 is applied to instruments and test carriers; Washer Disinfector Soil Test (BZ487) is spread in the chamber as a routine check.
How should it be stored, and how long does it keep?
Store it at 2–30 °C in the tightly closed container, protected from light and moisture. It is shipped at room temperature. Unopened, both versions have a 2-year shelf life.
Ordering Information
BZ489 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
