Saliva Artificial Test Soil ASTM F3208-20 (BZ482)

Saliva Artificial Test Soil ASTM F3208-20 – for Cleaning Validation of Oral Devices BZ482

Product Code: BZ482
Manufacturer: Biochemazone
Available Pack Sizes: Dried Powder: 5 g, 10 g, 20 g, 50 g – for making 50 mL, 100 mL, 200 mL, 500 mL · Premixed Ready to Use Formulation: 50 mL, 100 mL, 200 mL, 500 mL
Custom Bulk Quantities Available

Product Overview

Biochemazone Saliva Artificial Test Soil BZ482 is a simulated-use test soil formulated for cleaning validation of oral devices, in line with ASTM F3208-20. It mimics the contamination that builds up on a device in the mouth: purified bovine proteins (hemoglobin and albumin), polysaccharides, mucin and artificial saliva in a salt solution, with a pigment that gives the soil a red-brown colour. A hemoglobin-free version is available on request.

BZ482 is used for cleaning validation and verification of reusable oral devices such as mouthguards, dental trays and retainers, for comparing cleaning agents, detergents and automated cleaning systems, and in the development of cleaning technologies for oral healthcare. The formulation is non-hazardous and stable, so the same challenge can be repeated from study to study.

BZ482 can be ordered as Dried Powder or as a Premixed Ready to Use Formulation – the powder in 5 g, 10 g, 20 g and 50 g packs for making 50 mL, 100 mL, 200 mL and 500 mL, and the premixed formulation in 50 mL, 100 mL, 200 mL and 500 mL packs. The dried powder has a 2-year shelf life and can be reconstituted easily. Unopened, it has a 2-year shelf life at 2–30 °C and is shipped at room temperature. For surgical dental instruments see Dental Surgical Artificial Test Soil (BZ481); for devices that meet mucus see Simulated Mucus Test Soil (BZ483).

Two Versions to Select

• Dried Powder: Dry mixture that is reconstituted before the test.
• Premixed Ready to Use Formulation: Supplied mixed and ready to apply, with no weighing or mixing.

Select the version first and then the pack size. The Dried Powder has a 2-year shelf life and can be reconstituted easily: 5 grams is for making 50 mL, 10 grams is for making 100 mL, 20 grams is for making 200 mL and 50 grams is for making 500 mL. The Premixed Ready to Use Formulation is supplied in 50 mL, 100 mL, 200 mL and 500 mL packs.

Composition

• Purified bovine proteins: hemoglobin and albumin
• Polysaccharides and pigment
• Mucin and artificial saliva
• Salt solution
• Hemoglobin-free version available on request

How to Use

1. Prepare the soil: reconstitute the Dried Powder, or use the Premixed Ready to Use Formulation as supplied.
2. Soil the device: apply the test soil to the surfaces that sit in the mouth during use.
3. Clean: put the device through the cleaning procedure being validated.
4. Analyse: inspect for red-brown residue and measure residual protein against your acceptance criteria.

Key Features & Benefits

✅ Oral-Specific Simulation – Proteins, mucin and artificial saliva as found on oral devices
✅ ASTM F3208-20 – For simulated-use testing of reusable devices
✅ Hemoglobin-Free Option – Available on request
✅ Non-Hazardous – Stable formulation for reproducible results
✅ Two Versions to Select – Dried Powder (5 g to 50 g, for making 50 mL to 500 mL) or Premixed Ready to Use Formulation
✅ 2-Year Shelf Life – Unopened at 2–30 °C; shipped at room temperature
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

😁 Oral Devices
• Mouthguards
• Dental trays and retainers
• Other reusable oral instruments

🏥 Cleaning Validation
• Validation and verification studies
• Simulated-use soiling
• Reprocessing and care instructions

🧼 Cleaning Agents & Systems
• Detergent comparison
• Automated cleaning systems
• Cleaning tablets and solutions

🔬 Research & Development
• Cleaning technologies for oral healthcare
• New device materials
• Training

Typical Properties

• Composition: Purified bovine proteins (hemoglobin, albumin), polysaccharides, pigment, mucin, artificial saliva and salt solution
• Colour: Red-brown
• Variant: Hemoglobin-free version on request
• Standard: ASTM F3208-20
• Versions: Dried Powder; Premixed Ready to Use Formulation
• Dried Powder packs: 5 g, 10 g, 20 g, 50 g – for making 50 mL, 100 mL, 200 mL, 500 mL
• Premixed Ready to Use Formulation packs: 50 mL, 100 mL, 200 mL, 500 mL

Storage, Shelf Life & Transportation

• Storage Temperature: 2–30 °C, in the tightly closed container, protected from light and moisture
• Transportation: At room temperature
• Shelf Life: 2 years (unopened)
• Versions: Dried Powder and Premixed Ready to Use Formulation – the 2-year shelf life applies to both, unopened
• Dried Powder: 2-year shelf life and easy to reconstitute – 5 grams is for making 50 mL, 10 grams for 100 mL, 20 grams for 200 mL and 50 grams for 500 mL
• Protein-Containing Product: The Premixed Ready to Use Formulation is best kept at 2–8 °C for prolonged storage and stability; storage at 2–30 °C is fine for short-term use
• After Reconstitution or Opening: Refrigerate and use within two weeks; do not return unused soil to the container

Customization Options

Customization available on request:

• Hemoglobin-free version
• Custom formulations for a particular oral device
• Custom pack sizes and bulk quantities

Frequently Asked Questions

Which devices is BZ482 meant for?
Reusable devices that are worn or used in the mouth and meet saliva – mouthguards, dental trays, retainers and other oral instruments.

Is a version without hemoglobin available?
Yes. A hemoglobin-free version is supplied on request, for devices that do not meet blood or for assays in which hemoglobin interferes. State it in the order notes.

How does BZ482 differ from BZ481?
Dental Surgical Artificial Test Soil (BZ481) contains bone particulates and is meant for surgical dental instruments; BZ482 simulates saliva contamination on oral devices.

How should it be stored, and how long does it keep?
Store it at 2–30 °C in the tightly closed container, protected from light. It is shipped at room temperature, and unopened both versions have a 2-year shelf life. Because the soil contains proteins, the premixed version is best kept at 2–8 °C if it will be stored for a long time. Once the powder is reconstituted or the premixed soil is opened, refrigerate it and use it within two weeks.

Ordering Information

BZ482 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com

⚠️ For research, education, and in vitro testing only. Not for human or animal use.