British Standard Soil (BZ485)

British Standard Soil – Test Soil for Cleaning Validation of ENT and Other Reusable Medical Devices BZ485

Product Code: BZ485
Manufacturer: Biochemazone
Available Pack Sizes: Dried Powder: 5 g, 10 g, 20 g, 50 g – for making 50 mL, 100 mL, 200 mL, 500 mL · Premixed Ready to Use Formulation: 50 mL, 100 mL, 200 mL, 500 mL
Custom Bulk Quantities Available

Product Overview

Biochemazone British Standard Soil BZ485, also called British Soil (BRSO), is a standardized test soil that simulates clinically relevant organic contamination for cleaning validation and disinfectant efficacy studies. The blend combines physiological saline (0.9%), which gives the isotonic conditions of human tissue and blood; dry milk powder, a balanced source of proteins and carbohydrates; and dehydrated hog mucin, which adds a carbon source and the viscosity and tenacity of a biological soil.

BZ485 is particularly suited to devices exposed to mucus and blood, such as those used in ear, nose and throat (ENT) procedures – scopes, suction instruments and surgical tools. It can be used on its own or mixed with Defibrinated Blood Soil to simulate clinical soiling more closely, and it is used to validate cleaning protocols, to compare cleaning agents, detergents and automated cleaning systems, and to support validation work in line with ASTM F3208-20.

BZ485 can be ordered as Dried Powder or as a Premixed Ready to Use Formulation – the powder in 5 g, 10 g, 20 g and 50 g packs for making 50 mL, 100 mL, 200 mL and 500 mL, and the premixed formulation in 50 mL, 100 mL, 200 mL and 500 mL packs. The dried powder has a 2-year shelf life and can be reconstituted easily. Unopened, it has a 2-year shelf life at 2–30 °C and is shipped at room temperature. To add blood see Defibrinated Blood Soil; for a worst-case mucus soil see Simulated Mucus Test Soil (BZ483).

Two Versions to Select

• Dried Powder: Powder form, to be reconstituted before use.
• Premixed Ready to Use Formulation: Ready-to-use liquid form, with no weighing or mixing.

Select the version first and then the pack size. The Dried Powder has a 2-year shelf life and can be reconstituted easily: 5 grams is for making 50 mL, 10 grams is for making 100 mL, 20 grams is for making 200 mL and 50 grams is for making 500 mL. The Premixed Ready to Use Formulation is supplied in 50 mL, 100 mL, 200 mL and 500 mL packs.

Composition

• Physiological saline (0.9%)
• Dry milk powder – proteins and carbohydrates
• Dehydrated hog mucin – carbon source and viscosity
• Reddish-brown when combined with Defibrinated Blood Soil

How to Use

1. Prepare the soil: reconstitute the Dried Powder, or use the Premixed Ready to Use Formulation as supplied. For mucus-and-blood soiling, mix it with Defibrinated Blood Soil.
2. Soil the device: apply the test soil to the surfaces, joints and channels to be challenged.
3. Clean: put the device through the cleaning or disinfection procedure being evaluated.
4. Analyse: inspect the device and measure the residual soil against your acceptance criteria.

Key Features & Benefits

✅ Standardized Blend – Physiological saline, dry milk powder and dehydrated hog mucin
✅ ENT-Relevant – For devices exposed to mucus and blood
✅ Use Alone or with Blood – Compatible with Defibrinated Blood Soil
✅ Supports ASTM F3208-20 – For cleaning validation of reusable medical devices
✅ Two Versions to Select – Dried Powder (5 g to 50 g, for making 50 mL to 500 mL) or Premixed Ready to Use Formulation
✅ 2-Year Shelf Life – Unopened at 2–30 °C; shipped at room temperature
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

👂 ENT Instruments
• ENT scopes
• Suction instruments
• Surgical tools

🏥 Cleaning Validation
• Devices exposed to mucus and blood
• Cleaning verification studies
• Reprocessing instructions

🧴 Disinfectant Efficacy
• Organic soil load in disinfectant tests
• Surface and device studies
• Reproducible challenge matrix

🧼 Cleaning Agents & Systems
• Comparison of detergents
• Automated cleaning systems
• Optimizing cleaning protocols

Typical Properties

• Composition: Physiological saline (0.9%), dry milk powder and dehydrated hog mucin
• Colour: Reddish-brown when combined with Defibrinated Blood Soil
• Compatibility: Use alone or mixed with Defibrinated Blood Soil
• Versions: Dried Powder (reconstitute before use); Premixed Ready to Use Formulation
• Dried Powder packs: 5 g, 10 g, 20 g, 50 g – for making 50 mL, 100 mL, 200 mL, 500 mL
• Premixed Ready to Use Formulation packs: 50 mL, 100 mL, 200 mL, 500 mL

Storage, Shelf Life & Transportation

• Storage Temperature: 2–30 °C, in the tightly closed container, protected from light and moisture
• Transportation: At room temperature
• Shelf Life: 2 years (unopened)
• Versions: Dried Powder and Premixed Ready to Use Formulation – the 2-year shelf life applies to both, unopened
• Dried Powder: 2-year shelf life and easy to reconstitute – 5 grams is for making 50 mL, 10 grams for 100 mL, 20 grams for 200 mL and 50 grams for 500 mL
• Protein-Containing Product: The Premixed Ready to Use Formulation is best kept at 2–8 °C for prolonged storage and stability; storage at 2–30 °C is fine for short-term use
• After Reconstitution or Opening: Refrigerate and use within two weeks; do not return unused soil to the container

Customization Options

Customization available on request:

• Tailored blends for a particular test
• Combination with Defibrinated Blood Soil
• Custom pack sizes and bulk quantities

Frequently Asked Questions

What is British Soil made of?
Physiological saline (0.9%), dry milk powder and dehydrated hog mucin. The milk powder supplies proteins and carbohydrates, and the mucin gives the viscosity and tenacity of a biological soil.

Can it be used for disinfectant efficacy tests?
Yes. It is used as a standardized organic soil in cleaning validation and in disinfectant efficacy studies, where it represents the organic residues met in healthcare settings.

How do I simulate blood as well as mucus?
Mix BZ485 with Defibrinated Blood Soil; the combined soil is reddish-brown and represents the soiling of ENT procedures.

How should it be stored, and how long does it keep?
Store it at 2–30 °C in the tightly closed container, protected from light. It is shipped at room temperature, and unopened both versions have a 2-year shelf life. Because the soil contains proteins, the premixed version is best kept at 2–8 °C if it will be stored for a long time. Once the powder is reconstituted or the premixed soil is opened, refrigerate it and use it within two weeks.

Ordering Information

BZ485 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com

⚠️ For research, education, and in vitro testing only. Not for human or animal use.