1 M Tris-HCl Buffer, pH 8.8 – SDS-PAGE Resolving Gel Buffer (BZ216)

1 M Tris-HCl Buffer, pH 8.8, for SDS-PAGE Resolving Gels BZ216

Product Code: BZ216
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone 1 M Tris-HCl, pH 8.8 BZ216 is a buffer stock for the resolving (separating, lower) gel of SDS-PAGE and native PAGE. It sets the alkaline pH at which proteins separate by size after leaving the stacking gel.

In the Laemmli system the resolving gel is buffered at pH 8.8 with Tris-HCl at about 0.375 M final concentration. BZ216 supplies that buffer as a ready-made 1 M stock at pH 8.8 at 25 °C, saving weighing and titration. Labs that use the classic 4X recipe can choose 1.5 M Tris-HCl pH 8.8 (BZ201).

Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ 1 M Stock – For resolving gels
✅ pH 8.8 at 25 °C – Laemmli resolving-gel pH
✅ Reproducible Gels – Consistent separation from batch to batch
✅ Time-Saving – No weighing or HCl titration
✅ Pairs with pH 6.8 – Use with BZ206 stacking-gel buffer
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🧪 Protein Electrophoresis
• SDS-PAGE resolving (separating) gels
• Native-PAGE separating gels
• Gradient-gel preparation

🔬 Protein Analysis
• Western blot gels
• Purity and expression checks

🎓 Teaching Laboratories
• Protein electrophoresis practicals

Usage Areas

• Protein science and biochemistry laboratories
• Proteomics and Western blot groups
• Cell and molecular biology laboratories
• Biotechnology and pharmaceutical R&D
• Academic and teaching laboratories

Composition (per litre)

pH 8.8 at 25 °C. Typical use: 375 mL per litre of resolving-gel mix for 0.375 M final Tris-HCl.

Typical Properties

• Appearance: Clear, colourless liquid
• pH: 8.8 at 25 °C
• Concentration: 1 M Tris-HCl
• Buffering range of Tris: pH 7.0–9.0
• Temperature coefficient: About −0.03 pH units per °C

Available Formulations

• 1 M Tris-HCl pH 8.0, sterile (BZ214)
• 1 M Tris-HCl pH 7.4 (BZ217)
• 1 M Tris-HCl pH 6.8 (BZ206)
• 1 M Tris-HCl pH 8.8 (this product, BZ216)
• 1 M Tris-HCl pH 8.5, RNase-free (BZ215)
• 1.5 M Tris-HCl pH 8.8 (BZ201)
• 10 mM Tris-HCl pH 8.5 (BZ234)

Storage, Shelf Life & Transportation

• Storage Temperature: 2–30 °C
• Shelf Life: 1 year for unopened bottles
• Transportation: Room temperature (ambient shipping)
• Version with Preservatives: Available on request
• Powder Version: Available on request
• Keep tightly closed to prevent CO₂ uptake and pH drift

Customization Options

• Ready-made 4X resolving-gel buffer with 0.4% SDS
• 1.5 M or 2 M stocks
• Bulk containers

Ordering Information

BZ216 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.