Artificial Brain Fluid – Simulated Brain Fluid for Brain Slice and Neuroscience Research, pH 7.4 BZ256
Product Code: BZ256
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Artificial Brain Fluid BZ256, also called simulated brain fluid or cerebrospinal fluid simulation fluid, is a colourless, transparent, sterile liquid that simulates the human brain and cerebrospinal fluid environment, at pH 7.4. Its ion concentration, pH value and crystal osmotic pressure are like those of human brain and cerebrospinal fluid. It is sterilized through a 0.22 µm sterile filter membrane and can be used directly.
In scientific research, simulated brain fluid is used as a substitute for the natural fluid: it protects neurons during brain slice perfusion, and it is commonly used to maintain pH balance and tissue oxygen transport, in rat brain microdialysis experiments and in the culture of hippocampal tissue slices. For work at body temperature it is taken out half an hour in advance and warmed to 37 °C; other experiments follow the specific experiment plan.
It is supplied stabilized and has a 1-year shelf life unopened; a preservative-free version (6-month shelf life) and a dried Powder Version Kit are available on request. This simulated fluid is for scientific research and in vitro use only; it is not for clinical diagnosis or treatment. For the cerebrospinal solution see Artificial Cerebrospinal Solution (ACSF, Sterile) (BZ178); for brain fluid with mucus see Synthetic Brain Fluid with Mucus BZ541; for the brain interstitium see Artificial Cerebral Interstitial Fluid (BZ418).
Key Features & Benefits
✅ Simulates the Human Fluid – Ion concentration, pH and osmotic pressure like those of human brain and cerebrospinal fluid
✅ pH 7.4 – Customizable to the project requirement
✅ 0.22 µm Sterile-Filtered – Can be used directly
✅ Stabilized – Contains a preservative that stops microbial growth; 1-year shelf life unopened. Preservative-free version on request
✅ Sterile & Ready to Use – No weighing, mixing or pH adjustment before the test
✅ Reproducible & Standardized – Consistent batch-to-batch composition for routine QC and inter-laboratory comparison
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧠 Brain Slice Work
• Brain slice perfusion
• Culture of hippocampal tissue slices
🔬 Neuroscience Research
• Rat brain microdialysis experiments
• Maintaining pH balance and tissue oxygen transport
🧪 Laboratory Use
• Substitute for the natural fluid in in vitro experiments
• Reproducible replacement for collected fluid
Typical Properties
- Appearance: Colourless, transparent liquid
- pH: 7.4 (customizable)
- Ion concentration, pH and osmotic pressure: Like those of human brain and cerebrospinal fluid
- Sterility: Filtered through a 0.22 µm sterile membrane
- Format: Sterile, ready-to-use liquid, stabilized
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, protected from light
- Transportation: At room temperature
- Shelf Life: 1 year (unopened)
- Preservative-Free Version: Available on request – shelf life 6 months unopened at 2–30 °C
- Powder Version Kit: Available on request – 2-year shelf life, easy to reconstitute and suitable for culture-related applications; recommended when a preservative is not required
- After Opening: Handle aseptically; close tightly after use and do not return unused material to the container
Customization Options
Customization available on request:
- pH adjustment to the project requirement
- Contents to your specification
- Preservative-free (not-stabilized) version
- Powder Version Kit, custom pack sizes and bulk carboys (5 L, 10 L, 20 L)
Frequently Asked Questions
How is BZ256 prepared for use?
It is ready to use. For experiments at body temperature, take it out half an hour in advance and warm it to 37 °C; otherwise follow your experiment plan.
How long does the solution keep?
Unopened, the stabilized solution has a 1-year shelf life at 2–30 °C. A preservative-free version with a 6-month shelf life and a dried Powder Version Kit with a 2-year shelf life are available on request.
Ordering Information
BZ256 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
