Acetate Buffer (Acetic Acid–Sodium Acetate), pH 3.6–6.0 – Acidic Buffer for Enzyme Assays & Chromatography (BZ225)

Acetate Buffer (Acetic Acid–Sodium Acetate Buffer), pH 3.6–6.0 BZ225

Product Code: BZ225
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone Acetate Buffer BZ225 is a ready-to-use acetic acid–sodium acetate buffer – a weak acid paired with its salt – for work that needs a stable acidic pH between 3.6 and 6.0. The pH is set by the acetic acid : sodium acetate ratio, centred on the pK_a of acetic acid (4.76).

Acetate buffers are simple, inexpensive and UV-transparent at most working wavelengths. They are widely used for acid-pH enzyme assays, cation-exchange chromatography, protein and nanoparticle work at endosomal pH, staining procedures and as substrate buffers. The buffer is supplied at the pH you need within this range.

Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ pH 3.6–6.0 – Made to the pH you need in this range
✅ Classic Acetate System – Acetic acid + sodium acetate, pK_a 4.76
✅ Ready to Use – No weighing or titration
✅ Simple, Low-Interference – No phosphate, amines or detergents
✅ Versatile – Enzymes, chromatography, staining, drug release
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🧪 Biochemistry & Enzyme Assays
• Acid-pH enzyme assays (e.g. glycosidases, cellulases, acid phosphatases)
• Substrate buffers for colorimetric assays
• Protein precipitation and solubility screens

⚗️ Chromatography & Purification
• Cation-exchange chromatography buffers
• Elution and equilibration at acidic pH

💊 Pharmaceutical & Materials Research
• Drug-release studies at endosomal / lysosomal pH
• pH-responsive nanoparticle testing
• Histology and staining procedures (per protocol)

Usage Areas

• Biochemistry and enzymology laboratories
• Protein purification and analytical laboratories
• Pharmaceutical and drug-delivery R&D
• Food, plant and microbial research groups
• Academic and teaching laboratories

Composition (per litre)

Molarity and pH are made to your specification within pH 3.6–6.0 – please state the required pH and molarity in your order notes.

Typical Acetic Acid : Sodium Acetate Ratios (0.1 M buffer, 25 °C)

Reference values from the classic acetate buffer table; each batch is adjusted to the exact target pH.

Typical Properties

• Appearance: Clear, colourless liquid
• pH: 3.6–6.0 (to specification)
• Buffer: Acetic acid / sodium acetate (pK_a 4.76)
• Best buffering capacity: about pH 3.8–5.8

Available Formulations

• Acetate buffer pH 3.6–6.0 (this product, BZ225)
• Citrate buffer 0.1 M pH 4.5, sterile (BZ203)
• 0.1 M MES buffer (BZ229)
• Sodium citrate buffer powder pH 6.0 (BZ213)
• 0.1 M phosphate buffer pH 7.4, sterile (BZ204)

Storage, Shelf Life & Transportation

• Storage Temperature: 2–30 °C
• Shelf Life: 1 year for unopened bottles
• Transportation: Room temperature (ambient shipping)
• Version with Preservatives: Available on request
• Powder Version: Available on request
• Keep tightly closed; slight vinegar odour is normal
• Do not return unused buffer to the bottle

Customization Options

• Any molarity (e.g. 0.01–1 M) and pH 3.6–6.0
• Sterile-filtered version
• 3 M sodium acetate pH 5.2 (nucleic acid precipitation)
• Bulk containers

Ordering Information

BZ225 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.