0.1 M phosphate buffer pH 7.4, sterile sodium phosphate buffer, BZ204 – BioChemazone 500 mL bottle

0.1 M Phosphate Buffer, pH 7.4, Sterile – Sodium Phosphate Buffer (BZ204)

Price range: $15.00 through $39.00

0.1 M Phosphate Buffer, pH 7.4, Sterile (Sodium Phosphate Buffer) BZ204

Product Code: BZ204
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone 0.1 M Phosphate Buffer, pH 7.4 BZ204 is a sterile sodium phosphate buffer made from disodium hydrogen phosphate and sodium dihydrogen phosphate. It holds a near-physiological pH 7.4 at 25 °C and contains no sodium chloride, so it is ideal where a plain phosphate buffer – not PBS – is called for.

Phosphate buffers work best around pH 5.8–8.0, centred on the second pKa of phosphoric acid (about 7.2). The 0.1 M strength is the classic concentration for perfusion and fixative preparation (e.g. 4% paraformaldehyde in 0.1 M PB), tissue rinsing, enzyme assays and general dilution. The buffer is sterile-filtered through a 0.2 µm membrane.

Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ 0.1 M Sodium Phosphate – Classic PB strength, ready to use
✅ pH 7.4 at 25 °C – Near-physiological pH
✅ Saline-Free – No NaCl or KCl – not PBS
✅ Sterile-Filtered – 0.2 µm membrane filtration
✅ Fixative-Ready – Base buffer for 4% PFA and glutaraldehyde
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🔬 Histology & Neuroscience
• Preparing 4% paraformaldehyde (PFA) fixative in 0.1 M PB
• Transcardial perfusion rinse and fixation workflows
• Tissue rinsing and cryoprotection steps

🧪 Biochemistry
• Enzyme assays and kinetics at pH 7.4
• Protein dilution and dialysis
• Electron microscopy buffer preparation

🧫 Cell & Molecular Biology
• General-purpose neutral buffer
• Reagent and stain dilution

Usage Areas

  • Histology and pathology laboratories
  • Neuroscience and anatomy research groups
  • Biochemistry and enzymology laboratories
  • Electron microscopy facilities
  • Academic and teaching laboratories

Composition (per litre)

ComponentConcentrationAmount per litre
Disodium hydrogen phosphate heptahydrate (Na2HPO4·7H2O)0.0754 M20.21 g
Sodium dihydrogen phosphate monohydrate (NaH2PO4·H2O)0.0246 M3.39 g
High-purity water (ISO 3696)—to 1 L

0.1 M total phosphate, pH 7.4 at 25 °C. Contains no sodium chloride or potassium chloride.

Typical Properties

  • Appearance: Clear, colourless liquid
  • pH: 7.4 at 25 °C
  • Concentration: 0.1 M total phosphate
  • Saline: None (not PBS)
  • Format: Sterile-filtered (0.2 µm)

Available Formulations

  • 0.1 M phosphate buffer pH 7.4, sterile (this product, BZ204)
  • 1X PBS pH 7.4, sterile (BZ205)
  • 10X PBS concentrate (BZ220)
  • PBS powder (makes 1X) (BZ207)
  • DPBS 1X without Ca/Mg (BZ221)

Storage, Shelf Life & Transportation

  • Storage Temperature: 2–30 °C
  • Shelf Life: 1 year for unopened bottles
  • Transportation: Room temperature (ambient shipping)
  • Version with Preservatives: Available on request
  • Powder Version: Available on request
  • Phosphate may crystallise in the cold – warm to room temperature or 37 °C and mix until clear
  • After opening, handle aseptically and keep tightly closed

Customization Options

  • 0.01 M, 0.2 M or 0.5 M strengths
  • Any pH from 5.8 to 8.0
  • Potassium phosphate version
  • Bulk containers

Ordering Information

BZ204 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.

Description

Product overview

0.1 M Phosphate Buffer, pH 7.4 (BZ204) is a sterile, saline-free sodium phosphate buffer for fixative preparation, perfusion, enzyme assays and general laboratory use.

  • Catalogue no.: BZ204
  • Concentration: 0.1 M sodium phosphate
  • pH: 7.4 at 25 °C
  • Format: Sterile-filtered (0.2 µm), saline-free
  • Pack sizes: 100 mL, 200 mL, 500 mL, 1 L
  • Storage: 2–30 °C
  • Shelf life: 1 year (unopened bottle)
  • On request: Version with preservatives; powder version
  • Transportation: Room temperature
  • Use: Laboratory research use only

See also: 1X PBS pH 7.4 (BZ205) · 10X PBS (BZ220) · DPBS 1X (BZ221)

Frequently asked questions

What is the difference between phosphate buffer (PB) and PBS?

PB contains only sodium phosphate salts. PBS also contains sodium chloride and potassium chloride to make it isotonic. Many fixation and perfusion protocols ask for plain 0.1 M PB.

Can I make 4% PFA with this buffer?

Yes. 0.1 M PB pH 7.4 is the standard base for 4% paraformaldehyde fixative. Dissolve the PFA according to your protocol and cool before use.

The buffer has crystals after cold storage. Is it OK?

Yes. Phosphate salts can crystallise in the cold. Warm to room temperature or 37 °C and mix until fully clear.

Can I dilute it to 0.05 M or 0.01 M?

Yes. Dilute with high-purity water. The pH changes only slightly on dilution; check it if your method needs an exact value.

How should 0.1 M phosphate buffer be stored?

Store at 2–30 °C, tightly closed. Unopened bottles have a 1-year shelf life and ship at room temperature.

0.1 M Phosphate Buffer pH 7.4

0.1 M Phosphate Buffer pH 7.4 composition and customer’s specified configurations can be delivered upon request. Molarity, pH Value, Sodium or Potassium Salts, Container Size, and Contents of the buffer are customizable.
Contact us for Customization: info@biochemazone.com
Transportation and storage: Transportation at room temperature, storage at 2–30 ℃
We provide shipping facilities worldwide.

quality-assurance
Quality-Control-Approved
iso
iata
dubai-accreditation
iaf

Documents

Certificates of Analysis are issued per production batch. Please add the lot number or your order number to the request (catalogue no. BZ204 is filled in for you).

Additional information

Pack Size

100 mL, 200 mL, 500 mL, 1 L

Important Notes

  • Research Use Only: Biochemazone™ materials are intended strictly for research and development purposes in laboratory and industrial settings.
  • No Refunds or Exchanges: All sales are final. Biochemazone™ does not permit exchanges or offer refunds under any circumstances.
  • Customs & Import Responsibilities: Customs clearance, duties, taxes, and tariffs are the sole responsibility of the consignee/importer. Biochemazone™ is not liable for delays, rejections, or any additional costs incurred during the customs process, and such issues will not justify order cancellations or refunds.
  • Order Cancellations: Once an order is placed, it cannot be cancelled. No refunds will be issued for cancelled or undelivered orders.
  • Indemnification: The buyer agrees to indemnify and hold harmless Biochemazone™, its affiliates, and personnel from any claims, damages, liabilities, or expenses arising from the use of its products or services.

Terms & Conditions: All orders and communications are subject to Biochemazone™’s standard Terms & Conditions.

Disclaimer: Images and colors of the formulation displayed are for portrayal purposes only. Actual color and consistency may vary.

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