1X PBS phosphate buffered saline pH 7.4, sterile, BZ205 – BioChemazone 500 mL bottle

1X PBS (Phosphate Buffered Saline), pH 7.4, Sterile – Cell Culture Grade (BZ205)

Price range: $15.00 through $39.00

1X Phosphate Buffered Saline (PBS), 0.01 M, pH 7.2–7.4, Sterile BZ205

Product Code: BZ205
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone 1X Phosphate Buffered Saline (PBS) BZ205 is a ready-to-use, isotonic, sterile buffer for cell culture and general laboratory work. It holds cells, tissues and proteins at a stable pH of 7.2–7.4 and at physiological salt strength, which makes it the standard solution for washing cells, rinsing culture vessels, diluting reagents and carrying tissue samples.

The formulation is the classic 0.01 M phosphate buffer with 137 mM sodium chloride and 2.7 mM potassium chloride. It contains no calcium or magnesium, so it does not promote cell clumping and is ideal for rinsing adherent cells before trypsinisation. The solution is sterile and can be used straight from the bottle.

Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ Ready to Use – 1X working strength, no dilution needed
✅ pH 7.2–7.4 – Stable physiological pH for cells and proteins
✅ Isotonic – Matches the salt strength of body fluids
✅ Calcium- and Magnesium-Free – Ideal for rinsing cells before trypsinisation
✅ Sterile – Supplied sterile for aseptic cell culture work
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🧫 Cell Culture
• Washing and rinsing adherent and suspension cells
• Rinsing flasks and plates before trypsinisation
• Diluting cells for counting and passaging

🔬 Immunoassays & Staining
• Wash and dilution buffer for ELISA, immunocytochemistry and IHC
• Base buffer for blocking solutions and antibody diluents

🧪 General Laboratory Use
• Tissue rinsing and short-term transport
• Diluent for proteins, antibodies and reagents
• Base for PBS-Tween, PBS-BSA and PBS-EDTA

Usage Areas

  • Cell and tissue culture laboratories
  • Immunology and pathology laboratories
  • Biotechnology and biopharmaceutical R&D
  • Academic and teaching laboratories
  • Contract research organisations

Composition (1X, per litre)

ComponentConcentrationAmount per litre
Sodium chloride (NaCl)137 mM8.00 g
Potassium chloride (KCl)2.7 mM0.20 g
Disodium hydrogen phosphate (Na2HPO4)10 mM1.44 g
Potassium dihydrogen phosphate (KH2PO4)1.8 mM0.245 g
High-purity water (ISO 3696)—to 1 L

Calcium- and magnesium-free. pH adjusted to 7.2–7.4.

Typical Properties

  • Appearance: Clear, colourless liquid
  • pH: 7.2–7.4 at 25 °C
  • Concentration: 1X (0.01 M phosphate, 137 mM NaCl, 2.7 mM KCl)
  • Osmolality: Isotonic (typically about 280–310 mOsm/kg)
  • Calcium / Magnesium: None
  • Sterility: Sterile

Available Formulations

  • 1X PBS without calcium and magnesium (this product, BZ205)
  • 10X PBS concentrate (BZ220)
  • PBS powder (BZ207)
  • 1X PBS with 0.05% Tween 20 (BZ218)
  • Custom: with calcium and magnesium, with EDTA, or at another pH

Storage, Shelf Life & Transportation

  • Storage Temperature: 2–30 °C
  • Shelf Life: 1 year for unopened bottles
  • Transportation: Room temperature (ambient shipping)
  • Version with Preservatives: Available on request
  • Powder Version: Available on request
  • Keep tightly closed and use aseptic technique after opening
  • If crystals form after cold storage, warm to 37 °C and mix until dissolved

Customization Options

  • pH from 6.8 to 8.0
  • With calcium and magnesium
  • With Tween 20, BSA or EDTA added
  • Larger bulk containers

Ordering Information

BZ205 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.

Description

Product overview

1X Phosphate Buffered Saline (BZ205) is a sterile, ready-to-use, calcium- and magnesium-free PBS, 0.01 M, pH 7.2–7.4, for washing cells, rinsing tissues and diluting reagents.

  • Catalogue no.: BZ205
  • Concentration: 1X (0.01 M phosphate, 137 mM NaCl, 2.7 mM KCl)
  • pH: 7.2–7.4
  • Pack sizes: 100 mL, 200 mL, 500 mL, 1 L
  • Storage: 2–30 °C
  • Shelf life: 1 year (unopened bottle)
  • On request: Version with preservatives; powder version
  • Transportation: Room temperature
  • Use: Laboratory research use only

See also: 10X PBS (BZ220) · Dulbecco’s PBS (BZ221) · PBS Powder (BZ207)

Frequently asked questions

What is the pH of BZ205 PBS?

BZ205 is adjusted to pH 7.2–7.4 at 25 °C, the standard physiological pH used for cell culture, immunoassays and most biological work.

Does this PBS contain calcium or magnesium?

No. BZ205 is calcium- and magnesium-free, the preferred format for washing cells and rinsing cultures before trypsinisation. A version with calcium and magnesium can be made on request.

Is BZ205 sterile and ready to use?

Yes. It is a sterile 1X working solution and can be used directly from the bottle. Use aseptic technique after opening to keep it sterile.

How should PBS be stored and how long does it last?

Store at 2–30 °C. Unopened bottles have a shelf life of 1 year, and the product ships at room temperature. If crystals appear after cold storage, warm the bottle to 37 °C and mix until they dissolve.

What is the difference between PBS and DPBS?

Both are isotonic phosphate-buffered salines. Dulbecco’s PBS uses a slightly different phosphate balance and is widely used in cell culture. For most washing and dilution steps they are interchangeable. See DPBS BZ221.

1X PBS Phosphate Buffered Saline

1X PBS Phosphate Buffered Saline composition and customer’s specified configurations can be delivered upon request. Calcium/Magnesium Content, pH Value, Additives, Container Size, and Contents of the buffer are customizable.
Contact us for Customization: info@biochemazone.com
Transportation and storage: Transportation at room temperature, storage at 2–30 ℃
We provide shipping facilities worldwide.

quality-assurance
Quality-Control-Approved
iso
iata
dubai-accreditation
iaf

Documents

Certificates of Analysis are issued per production batch. Please add the lot number or your order number to the request (catalogue no. BZ205 is filled in for you).

Additional information

Pack Size

100 mL, 200 mL, 500 mL, 1 L

Important Notes

  • Research Use Only: Biochemazone™ materials are intended strictly for research and development purposes in laboratory and industrial settings.
  • No Refunds or Exchanges: All sales are final. Biochemazone™ does not permit exchanges or offer refunds under any circumstances.
  • Customs & Import Responsibilities: Customs clearance, duties, taxes, and tariffs are the sole responsibility of the consignee/importer. Biochemazone™ is not liable for delays, rejections, or any additional costs incurred during the customs process, and such issues will not justify order cancellations or refunds.
  • Order Cancellations: Once an order is placed, it cannot be cancelled. No refunds will be issued for cancelled or undelivered orders.
  • Indemnification: The buyer agrees to indemnify and hold harmless Biochemazone™, its affiliates, and personnel from any claims, damages, liabilities, or expenses arising from the use of its products or services.

Terms & Conditions: All orders and communications are subject to Biochemazone™’s standard Terms & Conditions.

Disclaimer: Images and colors of the formulation displayed are for portrayal purposes only. Actual color and consistency may vary.

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