KRBH Buffer (Krebs-Ringer Bicarbonate HEPES) with BSA, pH 7.4, Sterile – for Insulin Secretion Assays (BZ222)

KRBH Buffer (Krebs-Ringer Bicarbonate HEPES Buffer) with BSA, pH 7.4, Sterile BZ222

Product Code: BZ222
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone KRBH Buffer BZ222 is a sterile Krebs-Ringer bicarbonate HEPES buffer containing physiological salts, phosphate, a small amount of bicarbonate, HEPES, bovine serum albumin (BSA) and glucose. HEPES holds the pH near 7.4 even outside a CO₂ incubator, and BSA reduces non-specific loss of secreted peptides to plastic.

KRBH is the standard incubation buffer for glucose-stimulated insulin secretion (GSIS) assays on pancreatic islets and beta-cell lines, and is also used for glucose-uptake studies, adipocyte and muscle-cell assays and short-term tissue incubations. The buffer is sterile-filtered through a 0.2 µm membrane.

Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ Complete KRBH Formulation – Salts, phosphate, bicarbonate, HEPES, BSA, glucose
✅ HEPES-Stabilised pH 7.4 – Holds pH on the bench and in the incubator
✅ Contains BSA – Reduces peptide and hormone adsorption
✅ GSIS-Ready – Standard buffer for insulin secretion assays
✅ Sterile-Filtered – 0.2 µm membrane filtration
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🧬 Diabetes & Metabolism Research
• Glucose-stimulated insulin secretion (GSIS) on islets
• Beta-cell line assays (e.g. INS-1, MIN6, EndoC)
• Pre-incubation and starvation steps

🧪 Cell-Based Assays
• Glucose-uptake assays in adipocytes and muscle cells
• Hormone and peptide secretion studies
• Short-term metabolic incubations

🔬 Tissue Work
• Incubation and washing of isolated tissue
• Perfusion and tissue cleaning in vitro

Usage Areas

• Diabetes and islet biology laboratories
• Endocrinology and metabolism research groups
• Cell biology and pharmacology laboratories
• Biotechnology and pharmaceutical R&D
• Academic research laboratories

Typical Composition (per litre)

pH 7.4. This is the widely used KRBH formulation; batch values are given on the Certificate of Analysis. Glucose level (e.g. 2.8 mM basal or 16.7 mM stimulatory) and BSA content can be made to your specification.

Typical Properties

• Appearance: Clear to slightly opalescent, colourless liquid
• pH: 7.4
• Buffer: HEPES with bicarbonate and phosphate
• Protein: BSA, 0.1%
• Format: Sterile-filtered (0.2 µm)

Available Formulations

• KRBH buffer with BSA, sterile (this product, BZ222)
• Krebs-Henseleit buffer (BZ662)
• HEPES buffer (BZ228)
• HBSS 1X (Hank’s balanced salt solution) (BZ261)
• Ringer’s solution (BZ211)

Storage, Shelf Life & Transportation

• Storage Temperature: 2–30 °C
• Shelf Life: 1 year for unopened bottles
• Transportation: Room temperature (ambient shipping)
• Version with Preservatives: Available on request
• Powder Version: Available on request
• Contains BSA (protein): best kept at 2–8 °C for prolonged storage and performance; storage at 2–30 °C is fine for short-term use
• After opening, handle aseptically and keep tightly closed

Customization Options

• Basal (2.8 mM) and stimulatory (16.7 mM) glucose sets
• BSA-free or fatty-acid-free BSA versions
• Higher bicarbonate (Krebs-Ringer bicarbonate) versions
• Bulk containers

Ordering Information

BZ222 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.