Krebs-Henseleit Buffer (Organ-Bath and Perfusion Solution) BZ662
Product Code: BZ662
Manufacturer: Biochemazone
Available Pack Sizes: 500 mL, 1 L, 6 x 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Krebs-Henseleit Buffer BZ662 is the classic bicarbonate-buffered physiological salt solution used to keep isolated tissues alive and working. It is the standard perfusion fluid for the Langendorff isolated heart and the usual bath solution for aortic rings, small arteries, trachea, gut strips and other organ-bath preparations.
The solution contains sodium, potassium, calcium and magnesium chlorides and sulfate, potassium phosphate, sodium bicarbonate and glucose. When gassed with 95% oxygen / 5% carbon dioxide at 37 °C it has a pH of 7.4 (range 7.35–7.45). Without gassing the pH rises as carbon dioxide is lost, so gas the solution for at least 15–20 minutes before use. A calcium-free version is offered for tissue dissection and calcium-response curves, and a bicarbonate-free version for adding fresh bicarbonate on the day.
Calcium content varies between laboratories (commonly 1.25–2.5 mM); tell us your recipe and we will match it. The solution is sterile-filtered. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Classic Organ-Bath Solution – For hearts, vessels and smooth muscle
✅ pH 7.4 – When gassed with 95% O₂ / 5% CO₂
✅ Three Formulations – Complete, calcium-free, bicarbonate-free
✅ Sterile-Filtered – Low particle load for perfusion
✅ Recipe Matching – Calcium and glucose set to your protocol
✅ Up to 6 x 1 L – For daily perfusion work
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
❤️ Cardiac Physiology
• Langendorff isolated heart perfusion
• Working-heart preparations
• Ischaemia-reperfusion studies
🩸 Vascular Pharmacology
• Aortic and mesenteric ring myography
• Wire and pressure myography
🧪 Organ-Bath Studies
• Tracheal and bronchial rings
• Gut, bladder and uterine strips
Usage Areas
- Physiology and pharmacology laboratories
- Cardiovascular research groups
- Pharmaceutical safety-pharmacology units
- Contract research organisations
- Teaching laboratories
Typical Properties
- Appearance: Clear, colourless liquid
- pH: 7.4 (range 7.35–7.45) when gassed with 95% O₂ / 5% CO₂ at 37 °C
- Composition: Sodium, potassium, calcium and magnesium salts, phosphate, sodium bicarbonate and glucose in purified water
- Sterility: Sterile-filtered
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Gas with 95% O₂ / 5% CO₂ before use
Customization Options
- Calcium at your chosen level
- Added pyruvate or albumin
- 10x concentrates
- Dry powder that makes 10 L
Ordering Information
BZ662 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.
