0.1 M MES Buffer, pH 5.5 (2-(N-Morpholino)ethanesulfonic Acid Buffer) BZ229
Product Code: BZ229
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone 0.1 M MES Buffer, pH 5.5 BZ229 is a ready-to-use solution of MES (2-(N-morpholino)ethanesulfonic acid), one of the original Good’s buffers, titrated to pH 5.5 with sodium hydroxide.
MES has a pKa of about 6.1 at 25 °C and buffers well from pH 5.5 to 6.7. It contains no primary amines, phosphate or carboxylates and binds metal ions very weakly. That makes it the buffer of choice for EDC/NHS (carbodiimide) coupling of proteins, antibodies and peptides to beads, nanoparticles and surfaces, and a clean buffer for cation-exchange chromatography and slightly acidic enzyme work.
Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ 0.1 M MES – Ready to use
✅ pH 5.5 at 25 °C – Inside the MES buffering range (5.5–6.7)
✅ EDC/NHS-Compatible – No amines or carboxylates to compete
✅ Low Metal Binding – Suitable for metal-sensitive assays
✅ Good’s Buffer – Low UV absorbance, biologically inert
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
⚗️ Bioconjugation
• EDC/NHS activation of carboxyl beads and nanoparticles
• Antibody and protein coupling to surfaces
• Peptide and hapten conjugation
🧪 Chromatography & Biochemistry
• Cation-exchange chromatography buffers
• Enzyme assays at slightly acidic pH
• Protein crystallisation and stability screens
🌱 Plant & Cell Science
• pH buffering of plant culture media (per protocol)
• Slightly acidic buffer for cell and organelle work
Usage Areas
- Bioconjugation and diagnostics R&D
- Nanomaterials and surface-chemistry laboratories
- Protein purification laboratories
- Plant science laboratories
- Academic research laboratories
Composition (per litre)
| Component | Concentration | Amount per litre |
|---|
| MES (free acid) | 0.1 M | 19.5 g |
| Preservative | trace | — |
| Sodium hydroxide (NaOH) | to pH 5.5 | q.s. |
| High-purity water (ISO 3696) | — | to 1 L |
pH 5.5 at 25 °C. Contains a small amount of preservative (details on the Certificate of Analysis). For EDC/NHS coupling, many protocols use MES with 0.5 M NaCl – ask us for this version.
Typical Properties
- Appearance: Clear, colourless liquid
- pH: 5.5 at 25 °C
- Concentration: 0.1 M MES
- pKa: about 6.1 at 25 °C
- Buffering range: about pH 5.5–6.7
- Preservative: Present (see CoA)
Available Formulations
- 0.1 M MES buffer pH 5.5 (this product, BZ229)
- HEPES buffer 1X with Ca/Mg (BZ228)
- Acetate buffer pH 3.6–6.0 (BZ225)
- Borate buffered saline pH 8.0 (BZ224)
- Citrate buffer 0.1 M pH 4.5 (BZ203)
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Transportation: Room temperature (ambient shipping)
- Version with Preservatives: Available on request
- Powder Version: Available on request
- Keep tightly closed; a faint yellow tint on long storage does not usually affect buffering
- Do not return unused buffer to the bottle
Customization Options
- 0.05–0.5 M strengths
- Any pH from 5.5 to 6.7 (e.g. 6.0, 6.5)
- Preservative-free version
- MES-buffered saline (0.5 M NaCl) coupling buffer
Ordering Information
BZ229 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com
⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.
