Sodium Citrate Buffer Powder, 10 mM pH 6.0 (Antigen Retrieval / HIER Buffer) BZ213
Product Code: BZ213
Manufacturer: Biochemazone
Available Pack Sizes: Makes 1 L, 2 L, 5 L, 10 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Sodium Citrate Buffer Powder BZ213 is a pre-weighed dry blend that makes 10 mM sodium citrate buffer, pH 6.0 – the most widely used antigen retrieval buffer for immunohistochemistry (IHC) and immunocytochemistry. Simply dissolve each pack in deionised or distilled water to the stated volume.
Formaldehyde fixation forms cross-links that mask many epitopes. Heating sections in citrate buffer pH 6.0 (heat-induced epitope retrieval, HIER) reverses much of this masking and restores antibody binding on formalin-fixed paraffin-embedded (FFPE) and frozen sections. As a guide, 1 L of working buffer covers about 100 slides at 10 mL per slide. The dry format ships light, stores for 2 years and avoids weighing and pH adjustment.
Each batch is blended and packed under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Makes 10 mM Citrate, pH 6.0 – The standard HIER antigen retrieval buffer
✅ Pre-Weighed Packs – Make 1 L, 2 L, 5 L or 10 L
✅ No pH Adjustment – Dissolve and make up to volume
✅ 2-Year Shelf Life – Dry powder, unopened
✅ FFPE and Frozen Sections – Works with water bath, steamer, microwave or pressure cooker
✅ Light to Ship – Room-temperature dry format
✅ Cost-Effective – About 100 slides per litre at 10 mL per slide
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🔬 Immunohistochemistry (IHC)
• Heat-induced epitope retrieval (HIER) on FFPE sections
• Antigen retrieval for frozen and aldehyde-fixed sections
• Retrieval step before chromogenic or fluorescent IHC
🧫 Immunocytochemistry & Imaging
• Retrieval on fixed cell preparations
• Multiplex immunofluorescence workflows
🎓 Pathology Training
• Histology and IHC teaching laboratories
Usage Areas
- Histology and pathology research laboratories
- Cancer and tissue biomarker research groups
- Neuroscience and anatomy laboratories
- Biotechnology and pharmaceutical R&D
- Academic and teaching laboratories
Composition (per litre of prepared buffer)
| Component | Concentration | Amount per litre |
|---|
| Trisodium citrate dihydrate | ≈8.1 mM | ≈2.4 g |
| Citric acid monohydrate | ≈1.9 mM | ≈0.4 g |
| Total citrate | 10 mM | — |
| Deionised or distilled water (added by user) | — | to 1 L |
Gives pH 6.0 at 25 °C when dissolved to the stated volume. Optional: add 0.05% Tween 20 (0.5 mL per litre) if your protocol calls for it.
How to Use (HIER Guide)
| Step | What to do |
|---|
| 1. Prepare buffer | Dissolve one pack in about 90% of the final volume of water, then make up to the stated volume (1, 2, 5 or 10 L). |
| 2. Dewax and rehydrate | Dewax FFPE sections in xylene and rehydrate through graded ethanol to water. |
| 3. Heat | Heat slides in the buffer at 95–100 °C for 10–20 min (water bath, steamer, microwave or pressure cooker). |
| 4. Cool | Leave slides in the buffer to cool for about 20 min at room temperature. |
| 5. Continue IHC | Rinse in PBS or TBS wash buffer and continue with blocking and antibody steps. |
Optimise heating time and temperature for each antibody and tissue.
Typical Properties
- Appearance: White, free-flowing powder
- pH of prepared buffer: 6.0 at 25 °C
- Prepared concentration: 10 mM sodium citrate
- Format: Pre-weighed dry blend
Available Formulations
- Sodium citrate buffer powder, 10 mM pH 6.0 (this product, BZ213)
- Citrate buffer 0.1 M pH 4.5, sterile (liquid) (BZ203)
- TBS 0.05 M pH 7.4 (wash buffer) (BZ223)
- 10X TBST (BZ226)
- 10X PBST concentrate (BZ219)
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, tightly closed and dry
- Shelf Life: 2 years for unopened, sealed packs
- Transportation: Room temperature (ambient shipping)
- Keep packs sealed – the powder can absorb moisture
- Prepared buffer: keep tightly closed at 2–8 °C and use promptly; discard if cloudy
Customization Options
- Packs that make other volumes
- Ready-made liquid 10X or 1X citrate pH 6.0
- With 0.05% Tween 20 pre-blended
- EDTA or Tris-EDTA pH 9.0 retrieval powders
Ordering Information
BZ213 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com
⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.
