Physiological saline solution PHSS for skeletal muscle function with calcium, potassium and magnesium, sterile, BZ474 – BioChemazone 500 mL bottle

Physiological Saline Solution (PHSS) for Skeletal Muscle Function, Sterile – with Ca, K & Mg, pH 7.4 (BZ474)

Price range: $17.00 through $45.00

Physiological Saline Solution (PHSS) for Skeletal Muscle Function, Sterile BZ474

Product Code: BZ474
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone Physiological Saline Solution for Skeletal Muscle BZ474 is a sterile, ready-to-use salt solution that replicates the ionic composition of extracellular fluid. Alongside sodium chloride it contains calcium, potassium and magnesium – the ions skeletal muscle needs for normal excitation and contraction – at a physiological pH of 7.4 ± 0.2.

It is designed to keep isolated skeletal muscle viable and responsive in vitro and ex vivo: for contraction, relaxation and fatigue studies, electrophysiological recordings, pharmacological testing and short-term preservation of excised muscle tissue.

Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ Muscle-Optimised Ions – Na+, K+, Ca2+, Mg2+
✅ pH 7.4 ± 0.2 – Physiological pH
✅ Sterile – Ready to use
✅ Contractility-Ready – For force, fatigue and relaxation studies
✅ Reproducible – Consistent batch-to-batch composition
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

💪 Muscle Physiology
• Ex vivo contraction, relaxation and fatigue studies
• Isolated muscle and fibre-bundle experiments
• Short-term preservation of excised muscle

⚡ Electrophysiology
• Muscle membrane and action-potential recordings
• Neuromuscular junction studies

💊 Pharmacology
• Drug-response and toxicity testing on muscle
• Biochemical and enzyme studies in muscle tissue

Usage Areas

  • Muscle physiology laboratories
  • Neuromuscular disease research groups
  • Pharmacology and toxicology laboratories
  • Exercise and metabolism research groups
  • Academic research laboratories

Composition

ComponentFunction
Sodium chloride (NaCl)Main extracellular ion, isotonicity
Potassium saltResting membrane potential
Calcium saltExcitation–contraction coupling
Magnesium saltMembrane and enzyme function
High-purity water (ISO 3696)Solvent

pH 7.4 ± 0.2. Batch concentrations are given on the Certificate of Analysis; custom ion levels are available.

Typical Properties

  • Appearance: Clear, colourless liquid
  • pH: 7.4 ± 0.2
  • Ions: Na+, K+, Ca2+, Mg2+, Cl−
  • Format: Sterile, ready to use

Available Formulations

  • PHSS for skeletal muscle (this product, BZ474)
  • Physiological saline 0.9% NaCl, sterile (BZ473)
  • Krebs-Henseleit buffer (BZ662)
  • Tyrode’s solution (BZ212)
  • Ringer’s solution (BZ211)

Storage, Shelf Life & Transportation

  • Storage Temperature: 2–30 °C
  • Shelf Life: 1 year for unopened bottles
  • Transportation: Room temperature (ambient shipping)
  • Version with Preservatives: Available on request
  • Powder Version: Available on request
  • Calcium salts can form a slight precipitate – warm gently and mix; discard if a heavy precipitate remains
  • After opening, handle aseptically and keep tightly closed

Customization Options

  • Custom Na, K, Ca and Mg levels
  • Calcium-free or high-potassium versions
  • With glucose, bicarbonate or HEPES buffer
  • Bulk containers

Ordering Information

BZ474 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.

Description

Product overview

Physiological Saline Solution for Skeletal Muscle (BZ474) is a sterile, ready-to-use saline with sodium, potassium, calcium and magnesium at pH 7.4 ± 0.2 for skeletal-muscle contractility, electrophysiology and tissue-preservation studies.

  • Catalogue no.: BZ474
  • Ions: Na+, K+, Ca2+, Mg2+
  • pH: 7.4 ± 0.2
  • Format: Sterile, ready to use
  • Pack sizes: 100 mL, 200 mL, 500 mL, 1 L
  • Storage: 2–30 °C
  • Shelf life: 1 year (unopened bottle)
  • On request: Version with preservatives; powder version
  • Transportation: Room temperature
  • Use: Laboratory research use only

See also: Krebs-Henseleit buffer (BZ662) · Physiological saline 0.9% (BZ473) · Ringer’s solution (BZ211)

Frequently asked questions

What is this solution used for?

For keeping isolated skeletal muscles viable and responsive during ex vivo contraction, fatigue, electrophysiology and pharmacology experiments.

Does it contain glucose or bicarbonate?

The standard version is a salt solution with sodium, potassium, calcium and magnesium. Glucose, bicarbonate or HEPES can be added on request for long organ-bath experiments.

At what temperature should I run experiments?

Isolated rodent muscles are often studied at 25–37 °C. Follow your protocol and keep the temperature constant.

How is it different from 0.9% saline?

Plain saline has only sodium chloride. This solution adds potassium, calcium and magnesium, which muscles need to contract and relax normally.

How should it be stored?

Store at 2–30 °C, tightly closed. Unopened bottles have a 1-year shelf life and ship at room temperature.

Physiological Saline Solution for Skeletal Muscle

Physiological Saline Solution for Skeletal Muscle composition and customer’s specified configurations can be delivered upon request. Ion Levels, Glucose, Buffer System, Additives, Container Size, and Contents of the solution are customizable.
Contact us for Customization: info@biochemazone.com
Transportation and storage: Transportation at room temperature, storage at 2–30 ℃
We provide shipping facilities worldwide.

quality-assurance
Quality-Control-Approved
iso
iata
dubai-accreditation
iaf

Documents

Certificates of Analysis are issued per production batch. Please add the lot number or your order number to the request (catalogue no. BZ474 is filled in for you).

Additional information

Pack Size

100 mL, 200 mL, 500 mL, 1 L

Important Notes

  • Research Use Only: Biochemazone™ materials are intended strictly for research and development purposes in laboratory and industrial settings.
  • No Refunds or Exchanges: All sales are final. Biochemazone™ does not permit exchanges or offer refunds under any circumstances.
  • Customs & Import Responsibilities: Customs clearance, duties, taxes, and tariffs are the sole responsibility of the consignee/importer. Biochemazone™ is not liable for delays, rejections, or any additional costs incurred during the customs process, and such issues will not justify order cancellations or refunds.
  • Order Cancellations: Once an order is placed, it cannot be cancelled. No refunds will be issued for cancelled or undelivered orders.
  • Indemnification: The buyer agrees to indemnify and hold harmless Biochemazone™, its affiliates, and personnel from any claims, damages, liabilities, or expenses arising from the use of its products or services.

Terms & Conditions: All orders and communications are subject to Biochemazone™’s standard Terms & Conditions.

Disclaimer: Images and colors of the formulation displayed are for portrayal purposes only. Actual color and consistency may vary.

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