TBS Buffer 0.05 M, pH 7.4 (Tris-Buffered Saline, 1X) BZ223
Product Code: BZ223
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone TBS Buffer 0.05 M, pH 7.4 BZ223 is a ready-to-use, isotonic Tris-buffered saline of 50 mM Tris-HCl and 150 mM sodium chloride. It is the classic wash and dilution buffer for immunohistochemistry, in situ hybridisation, ELISA and Western blotting.
Because it contains no phosphate, TBS is the preferred buffer when working with alkaline phosphatase (AP) conjugates or detecting phospho-proteins, where phosphate can interfere. Add Tween 20 or Triton X-100 to make TBST or TBS-T washes, or BSA or milk for blocking.
Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ 50 mM Tris, 150 mM NaCl – Classic isotonic TBS, ready to use
✅ pH 7.4 at 25 °C – Physiological working pH
✅ Phosphate-Free – Ideal for AP conjugates and phospho-proteins
✅ Versatile Base – Add Tween 20, Triton X-100, BSA or milk
✅ 1X Working Strength – No dilution needed
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🔬 Immunohistochemistry & ISH
• Washing sections between antibody steps
• In situ hybridisation washes
• Antibody and chromogen dilution
🧪 Immunoassays
• ELISA washing and dilution
• Western blot membrane washing (add Tween 20 for TBST)
• Blocking-buffer base
🧫 General Biochemistry
• Protein dilution at neutral pH
• Phosphate-free alternative to PBS
Usage Areas
- Histology and pathology research laboratories
- Immunology and protein science laboratories
- Molecular biology and ISH groups
- Diagnostics R&D and assay development
- Academic and teaching laboratories
Composition (per litre)
| Component | Concentration | Amount per litre |
|---|
| Tris base (tris(hydroxymethyl)aminomethane) | 50 mM | 6.06 g |
| Sodium chloride (NaCl) | 150 mM | 8.77 g |
| Hydrochloric acid (HCl) | to pH 7.4 | q.s. |
| High-purity water (ISO 3696) | — | to 1 L |
pH 7.4 at 25 °C. Tris pH falls by about 0.03 units per °C rise, so check pH at your working temperature if exact pH matters.
Typical Properties
- Appearance: Clear, colourless liquid
- pH: 7.4 at 25 °C
- Concentration: 0.05 M Tris-HCl, 0.15 M NaCl (1X)
- Phosphate: None
- Temperature coefficient: About −0.03 pH units per °C
Available Formulations
- TBS 0.05 M pH 7.4 (this product, BZ223)
- 10X TBST (with Tween 20) (BZ226)
- 1 M Tris-HCl pH 7.4 (BZ217)
- 10X PBST concentrate (BZ219)
- Sodium citrate buffer powder pH 6.0 (antigen retrieval) (BZ213)
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Transportation: Room temperature (ambient shipping)
- Version with Preservatives: Available on request
- Powder Version: Available on request
- Keep tightly closed to prevent contamination and pH drift
- If crystals appear after cold storage, warm to room temperature and mix until clear
Customization Options
- 10X or 20X TBS concentrates
- pH 7.6 or 8.0 versions
- With Tween 20 or Triton X-100
- Bulk containers
Ordering Information
BZ223 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com
⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.
