Fasted State Simulated Intestinal Fluid (FaSSIF) – Biorelevant Dissolution Medium, Sterile, pH 6.50 BZ362
Product Code: BZ362
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Fasted State Simulated Intestinal Fluid (FaSSIF) BZ362 is a sterile, ready-to-use biorelevant dissolution medium at pH 6.50 ± 0.1 that simulates the components and pH of the fluid of the upper intestine before a meal. It contains a bile salt (sodium taurocholate) and lecithin, following published FaSSIF recipes, with the enzymes trypsin and pancreatin, and is sterilized by 0.2 µm filtration.
FaSSIF is a very useful drug development tool. Tests in this medium help to show how an oral drug is likely to dissolve, and potentially be absorbed, in the fluid of the upper intestine before a meal. The stability, solubility and dissolution data from FaSSIF help to identify the critical factors that influence a drug’s absorption in the fasted state. It is used with capsules, enteric-coated tablets and pills, especially enteric-coated capsules.
It is supplied stabilized and has a 1-year shelf life unopened; a preservative-free version (6-month shelf life) and a dried Powder Version Kit are available on request. Because it contains enzymes, keep it at 2–8 °C if it is to be stored for a long time. The composition, pH, viscosity and enzymes can be customized. This simulated fluid is for scientific research and in vitro use only; it is not for clinical diagnosis or treatment. For the other state see Fed State Simulated Intestinal Fluid (FeSSIF); for the powder see FaSSIF Powder Version (BZ551).
Key Features & Benefits
✅ Biorelevant FaSSIF – Sodium taurocholate and lecithin, following published recipes
✅ pH 6.50 ± 0.1 – Customizable to the project requirement
✅ With Enzymes – Trypsin and pancreatin; other enzyme options on request
✅ Stabilized – Contains a preservative that stops microbial growth; 1-year shelf life unopened. Preservative-free version on request
✅ Sterile-Filtered & Ready to Use – Sterilized by 0.2 µm filtration; no preparation before the test
✅ Reproducible & Standardized – Consistent batch-to-batch composition for routine QC and inter-laboratory comparison
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
💊 Drug Development
• Dissolution of oral drugs in the fasted state
• Stability and solubility testing
• Factors that influence absorption in the fasted state
🔬 Dosage Form Research
• Capsules and enteric-coated capsules
• Enteric-coated tablets and pills
Typical Properties
- Appearance: Clear to hazy with stringy fibres, colourless, characteristic odour
- pH: 6.50 ± 0.1 at 25 °C (customizable)
- Key components: Bile salt (sodium taurocholate) and lecithin; trypsin and pancreatin
- Sterility: Sterilized by 0.2 µm filtration
- Format: Sterile, ready-to-use liquid, stabilized
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, protected from light
- Transportation: At room temperature
- Shelf Life: 1 year (unopened)
- Preservative-Free Version: Available on request – shelf life 6 months unopened at 2–30 °C
- Enzyme Versions: Best kept at 2–8 °C for prolonged storage and activity; storage at 2–30 °C is fine for short-term use
- Powder Version Kit: Available on request – 2-year shelf life, easy to reconstitute and suitable for culture-related applications; recommended when a preservative is not required
- After Opening: Handle aseptically; close tightly after use and do not return unused material to the container
Customization Options
Customization available on request:
- pH adjustment to the project requirement
- Enzyme customization
- Composition and viscosity
- Preservative-free (not-stabilized) version
- Powder Version Kit, custom pack sizes and bulk carboys (5 L, 10 L, 20 L)
Frequently Asked Questions
What is FaSSIF used for?
It is a dissolution medium that represents the fluid of the upper small intestine before a meal. Testing an oral drug in it shows how the drug is likely to dissolve in the fasted state.
How should it be stored?
Store it at 2–30 °C; because it contains enzymes, 2–8 °C is best for prolonged storage and to keep the enzymes active. It is shipped at room temperature. Unopened, the stabilized solution has a 1-year shelf life; a preservative-free version with a 6-month shelf life and a dried Powder Version Kit with a 2-year shelf life are available on request.
Ordering Information
BZ362 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
