Simulated Urine or Artificial Urine solution is designed in such a way that supports the growth of various urinary tract pathogens and it has pH 6.5. Our Artificial Urine formulation has a similar composition and ingredients that simulate the biological contents, mineral composition, enzymes, and pH of natural urine. This ready-to-use formulation mimics real human urine composition. It is colorless to light yellow. This formulation can be stored at room temperature and has a pH of 6.5. This solution is only intended to be used for clinical studies as well as for product testing and research, and not for in vivo medical use.

Buy premium quality simulated urine solution at Low price. The pH of the solution is 6.5 Ask for the customization, (Custom pH versions also available)

pH and contents are customizable upon request.

Application: supports growth of urinary pathogens and crystals formation study.

​Artificial Urine Medium (AUM) is a synthetic medium that closely mimics the composition of human urine, making it suitable for the growth of urological pathogens. Here are the typical components and the rationale behind their inclusion in such a medium:

Composition of Artificial Urine Medium (AUM)

1. Urea: A primary component of urine that provides a nitrogen source.
2. Creatinine: Another nitrogenous waste product found in urine.
3. Uric Acid: A minor component of urine, serving as a nitrogen source.
4. Electrolytes: Such as sodium chloride, potassium chloride, calcium chloride, and magnesium sulfate to replicate the ionic composition of urine.
5. Organic Acids: Including citric acid and lactic acid, which are present in trace amounts in urine.
6. Phosphates: Such as monobasic sodium phosphate and dibasic sodium phosphate, which act as buffering agents.
7. Vitamins and Trace Elements: Including B-vitamins and trace metals to support microbial growth.
8. Carbohydrates: Small amounts of glucose or other sugars to provide energy sources.
9. Amino Acids and Peptides: To support the growth of fastidious organisms.

Preparation

1. Mixing: All components are dissolved in distilled water, ensuring complete dissolution and proper pH adjustment to closely match that of natural urine (typically around pH 6.5).
2. Sterilization: The medium is sterilized, usually by filtration, to ensure that it is free of contaminants before use in microbial culture.

Applications

1. Pathogen Cultivation: Used to grow and study urological pathogens like Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and others.
2. Antimicrobial Testing: Suitable for assessing the efficacy of antibiotics and other antimicrobial agents against urological pathogens.
3. Biofilm Studies: Ideal for studying biofilm formation by urological pathogens in a controlled environment.
4. Genetic and Physiological Studies: Allows for the investigation of the genetic and physiological responses of pathogens to conditions mimicking those found in the urinary tract.

Example Pathogens Grown in AUM

• Escherichia coli: Often responsible for urinary tract infections (UTIs).
• Klebsiella pneumoniae: Known for causing UTIs and other healthcare-associated infections.
• Proteus mirabilis: Noted for its ability to form crystals and cause catheter-associated UTIs.
• Enterococcus faecalis: A common cause of complicated UTIs.

If you need the specific formulation for a commercial or research application, it would typically be found in scientific publications or product datasheets from suppliers of microbiological media.