Artificial Sweat – JIS L 0848:1978 Method D, Acidic Artificial Perspiration (pH 5.5) BZ564
Product Code: BZ564
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Artificial Sweat JIS L 0848:1978, Method D-Acidic (pH 5.5) BZ564 is a ready-to-use acidic artificial perspiration prepared to the Method D acidic test solution of the Japanese Industrial Standard JIS L 0848:1978 (test method for colour fastness to perspiration). The solution is an L-histidine / sodium chloride / sodium phosphate buffered solution adjusted to pH 5.5 – L-histidine monohydrochloride monohydrate, sodium chloride and sodium dihydrogen phosphate in purified water – reproducing the acidic sweat condition specified by the standard. JIS L 0848 Method D is supplied in two versions: Acidic (pH 5.5, BZ564) and Alkaline (pH 8.0, BZ566).
Prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system, each batch is standardised for composition and pH so that colour-fastness, corrosion and sweat-resistance results are reproducible and comparable between laboratories. It is intended for laboratories whose customer specifications, legacy test protocols or Japanese product standards call for the JIS L 0848:1978 Method D acidic perspiration solution.
BZ564 is used for colour-fastness and staining tests on textiles, dyes and leather, for corrosion and discolouration testing of plated parts, metal accessories, watch cases and bands, eyewear frames and wearable-device housings, and for sweat-resistance evaluation of coatings, adhesives, printed labels and consumer products. ISO 105-E04, AATCC 15, EN 1811, ISO 3160-2 and ISO 11641 artificial sweat formulations are also available.
Key Features & Benefits
✅ JIS L 0848:1978 Method D – Acidic Solution – Prepared to the Japanese perspiration-fastness standard
✅ Acidic pH 5.5 – Reproduces the acidic sweat condition of the Method D test solution
✅ L-Histidine / NaCl / Phosphate Buffered – Standard artificial perspiration composition, pH-adjusted with sodium hydroxide
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water standards
✅ Ready-to-Use Solution – No weighing, mixing or pH adjustment required
✅ Reproducible & Standardized – Consistent batch-to-batch composition for QC and comparative testing
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧵 Textile & Dye Testing
• Colour fastness to acidic perspiration per JIS L 0848
• Staining of adjacent / multifibre fabrics
• Leather, trims, prints and garment accessories
⌚ Metals, Coatings & Wearables
• Corrosion and discolouration of plated parts, jewellery and metal accessories
• Watch cases, bands, eyewear frames and wearable-device housings
• Sweat-resistance of coatings, anodised and painted finishes
🧴 Materials & Consumer Products
• Adhesives, printed labels and packaging in skin contact
• Elastomers, straps and grips
• Cosmetic and personal-care product testing
🔬 R&D and Quality Control
• Incoming material qualification against Japanese specifications
• Comparative testing between JIS, ISO, AATCC and EN artificial sweat formulations
Typical Properties
- Appearance: Colourless, clear solution
• pH: 5.5 ± 0.2 at 25 °C (acidic)
• Composition: L-histidine monohydrochloride monohydrate 0.5 g/L, sodium chloride 5 g/L, sodium dihydrogen phosphate dihydrate 2.2 g/L in purified water, adjusted to pH 5.5 with sodium hydroxide (JIS L 0848 acidic artificial perspiration)
• Water Grade: ISO 3696 purified water
• Format: Ready-to-use liquid; non-sterile
• Companion Product: Artificial Sweat JIS L 0848:1978, Method D-Alkaline (pH 8.0) BZ566
Storage & Stability
- Storage Temperature: 2–8 °C recommended; freeze at −20 °C for long-term storage
• Shelf Life: 6 months at 2–8 °C; 12 months when kept frozen
• Shipping: Stable at room temperature
Customization Options
- pH adjustment to customer specification
• ISO 105-E04, AATCC 15, EN 1811, ISO 3160-2 and ISO 11641 artificial sweat formulations
• Acetic acid / phosphate based Method D reference solution (pH 4.1) on request
• Sterile-filtered version, custom pack sizes and bulk / OEM supply
Ordering Information
BZ564 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
