CGS Buffer (Citrate-Glucose-Saline), pH 6.5, Sterile BZ233
Product Code: BZ233
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone CGS Buffer, pH 6.5 BZ233 is a sterile citrate-glucose-saline buffer used to wash platelets and other blood cells without activating them. It combines isotonic sodium chloride, sodium citrate and glucose at a mildly acidic pH 6.5.
Citrate binds calcium and the slightly acidic pH keeps platelets in a resting state, while glucose supports their metabolism. CGS is therefore a standard washing buffer when preparing washed platelets from platelet-rich plasma for aggregation, secretion, adhesion and signalling studies (see, for example, PMID 26808867). After washing, platelets are usually resuspended in a HEPES-buffered Tyrode’s buffer. The solution is sterile-filtered through a 0.2 µm membrane.
Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Citrate-Glucose-Saline – Classic platelet washing formulation
✅ pH 6.5 – Keeps platelets in a resting state
✅ Calcium-Chelating – Citrate limits activation and clumping
✅ With Glucose – Supports cell metabolism during washing
✅ Sterile-Filtered – 0.2 µm membrane filtration
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🩸 Platelet Research
• Washing platelets from platelet-rich plasma
• Preparing washed platelets for aggregometry
• Platelet secretion, adhesion and signalling studies
🔬 Blood-Cell Handling
• Gentle washing of blood cells in vitro
• Sample preparation for flow cytometry (per protocol)
🧪 Haemostasis Research
• Thrombosis and antiplatelet drug studies
• Coagulation and haematology method development
Usage Areas
- Platelet and haemostasis research laboratories
- Haematology and thrombosis research groups
- Cardiovascular pharmacology laboratories
- Biotechnology and pharmaceutical R&D
- Academic research laboratories
Typical Composition (per litre)
| Component | Concentration | Amount per litre |
|---|
| Sodium chloride (NaCl) | 120 mM | 7.01 g |
| Trisodium citrate dihydrate | 12.9 mM | 3.79 g |
| D-Glucose | 30 mM | 5.41 g |
| High-purity water (ISO 3696) | — | to 1 L |
pH 6.5 at 25 °C. Widely used CGS formulation; batch values are given on the Certificate of Analysis.
Typical Properties
- Appearance: Clear, colourless liquid
- pH: 6.5 at 25 °C
- Anticoagulant: Sodium citrate (calcium-chelating)
- Format: Sterile-filtered (0.2 µm)
Available Formulations
- CGS buffer pH 6.5, sterile (this product, BZ233)
- Modified Tyrode’s solution (HEPES-buffered) (BZ235)
- Tyrode’s solution (BZ212)
- Citrate buffer 0.1 M pH 4.5 (BZ203)
- 20X SSC (saline-sodium citrate) (BZ231)
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Transportation: Room temperature (ambient shipping)
- Version with Preservatives: Available on request
- Powder Version: Available on request
- Glucose-containing solutions support microbial growth once contaminated – handle aseptically
- Bring to the temperature required by your protocol before use
Customization Options
- Other NaCl, citrate or glucose levels
- ACD (acid-citrate-dextrose) anticoagulant
- With prostaglandin-free or EDTA variants on request
- Bulk containers
Ordering Information
BZ233 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com
⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.
