Citrate buffer 0.1 M pH 4.5, sterile citric acid–sodium citrate buffer, BZ203 – BioChemazone 500 mL bottle

Citrate Buffer 0.1 M, pH 4.5, Sterile (Citric Acid–Sodium Citrate) (BZ203)

Price range: $19.00 through $55.00

Citrate Buffer 0.1 M, pH 4.5, Sterile (Citric Acid–Sodium Citrate Buffer) BZ203

Product Code: BZ203
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone Citrate Buffer 0.1 M, pH 4.5 BZ203 is a sterile, ready-to-use citric acid–sodium citrate buffer for work that needs a stable, mildly acidic pH. Total citrate is 0.1 mol/L and the pH is set to 4.5 at 25 °C by the citric acid : sodium citrate ratio, so no strong acid or base is added.

Citrate buffers best between about pH 3.0 and 6.2. At pH 4.5 the buffer sits close to the second pKa of citric acid (about 4.8), which gives good capacity. The solution is sterile-filtered through a 0.2 µm membrane and filled into clean bottles, ready for acidic-pH enzyme assays, drug-release studies at lysosomal pH and preparing acid-labile reagents such as streptozotocin (STZ).

Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ 0.1 M Total Citrate – Ready to use, no dilution needed
✅ pH 4.5 at 25 °C – Close to the citrate pKa for good buffering
✅ Sterile-Filtered – 0.2 µm membrane filtration
✅ No Strong Acid Added – pH set by the citric acid : citrate ratio
✅ Acidic-pH Workhorse – Enzyme assays, drug release, STZ preparation
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🧪 Biochemistry & Enzyme Assays
• Acid-pH enzyme assays (e.g. cellulases, pectinases, acid hydrolases)
• Substrate and reagent dilution at pH 4.5
• Protein stability and solubility checks at acidic pH

💊 Pharmaceutical & Nanomedicine Research
• In vitro drug-release studies at lysosomal / endosomal pH
• pH-responsive nanoparticle and hydrogel testing
• Acidic dissolution and stability screening

🔬 Biomedical Research
• Preparing streptozotocin (STZ) solutions in diabetes research protocols
• Diluting acid-labile reagents immediately before use

Usage Areas

  • Biochemistry and enzymology laboratories
  • Pharmaceutical and drug-delivery R&D
  • Diabetes and metabolic research groups
  • Food, plant and microbial enzyme laboratories
  • Academic and teaching laboratories

Composition (per litre)

ComponentConcentrationAmount per litre
Citric acid monohydrate≈0.054 M≈11.2 g
Trisodium citrate dihydrate≈0.046 M≈13.7 g
Total citrate0.1 M—
High-purity water (ISO 3696)—to 1 L

pH 4.5 at 25 °C. The citric acid : sodium citrate ratio is fine-tuned per batch to reach the target pH; amounts shown are typical.

Typical Properties

  • Appearance: Clear, colourless liquid
  • pH: 4.5 at 25 °C
  • Concentration: 0.1 M total citrate
  • Buffering range of citrate: about pH 3.0–6.2
  • Format: Sterile-filtered (0.2 µm)

Available Formulations

  • Citrate Buffer 0.1 M, pH 4.5, sterile (this product, BZ203)
  • Sodium citrate buffer powder, 10 mM pH 6.0 (antigen retrieval) (BZ213)
  • Acetic acid–sodium acetate buffer, pH 3.6–6.0 (BZ225)
  • 20X SSC (saline-sodium citrate), pH 7.0 (BZ231)
  • 0.1 M phosphate buffer pH 7.4, sterile (BZ204)

Storage, Shelf Life & Transportation

  • Storage Temperature: 2–30 °C
  • Shelf Life: 1 year for unopened bottles
  • Transportation: Room temperature (ambient shipping)
  • Version with Preservatives: Available on request
  • Powder Version: Available on request
  • After opening, handle aseptically and keep tightly closed
  • Citrate solutions can support mould growth once contaminated – do not return unused buffer to the bottle

Customization Options

  • Other molarities (0.01–0.5 M)
  • Any pH from 3.0 to 6.2
  • Non-sterile or bulk containers
  • Citrate-phosphate (McIlvaine) buffers

Ordering Information

BZ203 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human use, clinical diagnosis or therapeutic use.

Description

Product overview

Citrate Buffer 0.1 M, pH 4.5 (BZ203) is a sterile, ready-to-use citric acid–sodium citrate buffer for acidic-pH enzyme assays, drug-release studies and STZ solution preparation.

  • Catalogue no.: BZ203
  • Concentration: 0.1 M total citrate
  • pH: 4.5 at 25 °C
  • Format: Sterile-filtered (0.2 µm)
  • Pack sizes: 100 mL, 200 mL, 500 mL, 1 L
  • Storage: 2–30 °C
  • Shelf life: 1 year (unopened bottle)
  • On request: Version with preservatives; powder version
  • Transportation: Room temperature
  • Use: Laboratory research use only

See also: Sodium citrate buffer powder pH 6.0 (BZ213) · Acetate buffer pH 3.6–6.0 (BZ225) · 0.1 M phosphate buffer pH 7.4 (BZ204)

Frequently asked questions

Why use 0.1 M citrate buffer at pH 4.5?

pH 4.5 is close to the second pKa of citric acid, so the buffer holds its pH well. It is a common choice for acidic enzyme assays and drug-release tests at lysosomal pH.

Can I use BZ203 to dissolve streptozotocin (STZ)?

Yes. 0.1 M citrate pH 4.5 is the buffer most often used in research protocols for STZ. STZ breaks down quickly in solution, so dissolve it in cold buffer right before use.

Is the buffer sterile?

Yes. It is filtered through a 0.2 µm membrane. Once opened, handle it aseptically to keep it sterile.

Can I dilute it to 0.05 M?

Yes. Dilute 1:1 with high-purity water. The pH shifts only slightly; check it if your method needs an exact value.

How should 0.1 M citrate buffer pH 4.5 be stored?

Store at 2–30 °C, tightly closed. Unopened bottles have a 1-year shelf life and ship at room temperature.

Citrate Buffer 0.1 M pH 4.5

Citrate Buffer 0.1 M pH 4.5 composition and customer’s specified configurations can be delivered upon request. Molarity, pH Value, Sterility, Container Size, and Contents of the buffer are customizable.
Contact us for Customization: info@biochemazone.com
Transportation and storage: Transportation at room temperature, storage at 2–30 ℃
We provide shipping facilities worldwide.

quality-assurance
Quality-Control-Approved
iso
iata
dubai-accreditation
iaf

Documents

Certificates of Analysis are issued per production batch. Please add the lot number or your order number to the request (catalogue no. BZ203 is filled in for you).

Additional information

Pack Size

100 mL, 200 mL, 500 mL, 1 L

Important Notes

  • Research Use Only: Biochemazone™ materials are intended strictly for research and development purposes in laboratory and industrial settings.
  • No Refunds or Exchanges: All sales are final. Biochemazone™ does not permit exchanges or offer refunds under any circumstances.
  • Customs & Import Responsibilities: Customs clearance, duties, taxes, and tariffs are the sole responsibility of the consignee/importer. Biochemazone™ is not liable for delays, rejections, or any additional costs incurred during the customs process, and such issues will not justify order cancellations or refunds.
  • Order Cancellations: Once an order is placed, it cannot be cancelled. No refunds will be issued for cancelled or undelivered orders.
  • Indemnification: The buyer agrees to indemnify and hold harmless Biochemazone™, its affiliates, and personnel from any claims, damages, liabilities, or expenses arising from the use of its products or services.

Terms & Conditions: All orders and communications are subject to Biochemazone™’s standard Terms & Conditions.

Disclaimer: Images and colors of the formulation displayed are for portrayal purposes only. Actual color and consistency may vary.

Related products