Tripartite Soil Load for ASTM E2197 (Bovine Serum Albumin, Yeast Extract and Mucin) BZ584
Product Code: BZ584
Manufacturer: Biochemazone
Available Pack Sizes: Kits of three bottles: 3 × 10 mL, 3 × 25 mL, 3 × 50 mL
Custom Bulk Quantities Available
Product Overview
Biochemazone Tripartite Soil Load BZ584 is the three-part organic soil used in ASTM E2197, the quantitative disk carrier test for bactericidal, virucidal, fungicidal, mycobactericidal and sporicidal activity of liquid chemicals. The soil represents the mix of large proteins, small peptides and mucus that a disinfectant meets on real surfaces.
The kit contains three sterile stock solutions in phosphate-buffered saline: bovine serum albumin (50 mg/mL), yeast extract (50 mg/mL) and bovine mucin (4 mg/mL). In the method, 25 µL BSA, 35 µL yeast extract and 100 µL mucin are combined with 340 µL of the test organism suspension. The solutions have a pH of about 7.2 (range 7.0–7.4).
Using ready-made, matched stocks keeps the soil load identical from run to run and saves weighing and sterilising three separate components. The soil load is also used in other carrier methods that ask for a three-part soil, including testing for EPA and Health Canada disinfectant submissions. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Made for ASTM E2197 – Three-part soil load for the quantitative disk carrier test
✅ Three Matched Components – BSA 50 mg/mL, yeast extract 50 mg/mL, mucin 4 mg/mL
✅ Sterile Stocks – Each component prepared sterile
✅ pH About 7.2 – In phosphate-buffered saline
✅ Pre-Measured Concentrations – Pipette straight into the 25 + 35 + 100 µL soil mix
✅ Easy Storage – 2–30 °C; 1 year shelf life unopened
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧫 Carrier Efficacy Testing
• ASTM E2197 quantitative disk carrier test
• Bactericidal, virucidal, fungicidal and sporicidal claims
• Carrier tests that specify a three-part soil load
📋 Regulatory Testing
• Data for EPA and Health Canada disinfectant submissions
• Hard-surface and medical-device disinfectant claims
• Hospital disinfectant product development
🔬 Research
• Soil-load effect studies on disinfectant chemistry
• Neutraliser and recovery validation
• Training of efficacy-testing staff
Usage Areas
- Disinfectant and sterilant manufacturers
- GLP and accredited efficacy-testing laboratories
- Regulatory consultants for antimicrobial products
- Medical-device reprocessing researchers
- Universities and research institutes
How to Use
- Bring the three bottles to room temperature and mix each gently.
- For each carrier test, combine 25 µL BSA, 35 µL yeast extract and 100 µL mucin.
- Add 340 µL of the test organism suspension and mix.
- Use the soil-loaded inoculum as described in the test method.
Typical Properties
- Appearance: Clear to slightly opalescent liquids
- pH: about 7.2 (range 7.0–7.4)
- Bovine serum albumin: 50 mg/mL in PBS
- Yeast extract: 50 mg/mL in PBS
- Bovine mucin: 4 mg/mL in PBS
- Water Grade: ISO 3696 purified water
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Best kept at 2–8 °C for prolonged storage and stability of the protein (BSA, yeast extract and mucin) components; storage at 2–30 °C is fine for short-term use
- Mix gently before use
Customization Options
- Single components supplied separately
- Pre-mixed soil load
- Pre-aliquoted single-use tubes
- Custom volumes and bulk supply
Ordering Information
BZ584 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For laboratory testing only. Not for human or animal use.
