Probiotic Acid and Bile Tolerance Test Fluids BZ604
Product Code: BZ604
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L per fluid, or kits of all three
Custom Bulk Quantities Available
Product Overview
Biochemazone Probiotic Acid and Bile Tolerance Test Fluids BZ604 are ready-made challenge fluids for showing whether probiotic strains and finished products survive passage through the stomach and small intestine. Acid and bile tolerance are among the first screening tests for a probiotic candidate and are routinely used to compare strains, encapsulation and capsule designs.
Three fluids are offered. The acid challenges are simulated gastric fluids with pepsin at pH 2.0 (a harsh fasting stomach) and pH 3.0 (a milder or fed stomach). The bile challenge contains 0.3% oxgall (dried bovine bile) at pH 7.0, the bile level most often used in the literature. They can be used alone or in sequence: acid first, then bile.
All fluids are sterile and ready to use, and are prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system. Oxgall is of animal origin.
Key Features & Benefits
✅ Two Acid Levels – pH 2.0 and pH 3.0, with pepsin
✅ Bile Challenge – 0.3% oxgall at pH 7.0
✅ Sequential Testing – Acid then bile, as in transit through the gut
✅ Sterile – No contaminating organisms in viability counts
✅ Kit Option – All three fluids together
✅ Ready-to-Use – No weighing of pepsin or bile
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🦠 Probiotic Strain Screening
• Acid tolerance of lactobacilli, bifidobacteria and other candidates
• Bile tolerance and bile-salt resistance
• Comparison of wild-type and adapted strains
💊 Product Development
• Encapsulation, microencapsulation and enteric-capsule testing
• Survival in finished foods and supplements
• Shelf-life and formulation comparisons
🔬 Research & Teaching
• Food-microbiology research
• Student practicals on gut survival
Usage Areas
- Probiotic and supplement manufacturers
- Dairy and functional-food companies
- Food-microbiology laboratories
- Contract testing laboratories
- Universities and research institutes
Typical Properties
- Appearance: Clear to slightly hazy (acid fluids); yellow-green (bile fluid)
- pH: acid challenges 2.0 and 3.0; bile challenge 7.0
- Acid challenges: Sodium chloride, hydrochloric acid and pepsin
- Bile challenge: 0.3% (w/v) oxgall in buffered saline
- Sterility: Sterile
- Water Grade: ISO 3696 purified water
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Best kept at 2–8 °C for prolonged storage and stability of the pepsin and oxgall bile components; storage at 2–30 °C is fine for short-term use
- Warm to 37 °C before use
Customization Options
- Other pH values (for example 1.5 or 2.5) and bile concentrations (0.5–2%)
- Defined bile-salt mixtures instead of oxgall
- Pancreatin-containing intestinal challenge
- Custom pack sizes and bulk supply
Ordering Information
BZ604 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research use only. Not for human or animal use.
