Artificial Cytosolic Fluid – Synthetic Intracellular Fluid (Cytosol) for In Vitro Research, pH 7.2 BZ495
Product Code: BZ495
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Artificial Cytosolic Fluid BZ495, also called synthetic cytosolic fluid, is a sterile, ready-to-use solution that mimics the composition and physicochemical properties of the intracellular fluid (cytosol) found in human and animal cells. It replicates the ionic strength, pH and solute composition of natural cytosolic fluid. The standard formulation is set at pH 7.2, within the range 7.2 to 7.4, and the pH can be customized to the project requirement.
The standard formulation contains the electrolytes sodium, potassium, magnesium, calcium, chloride, phosphate and sulfate, with glucose, amino acids and reducing agents such as GSH, in a HEPES- or Tris-based buffer; ATP precursors are optional. BZ495 is used to simulate cellular conditions in vitro: in cell biology and biochemistry for studies of cellular metabolism, signalling pathways and cytosolic protein interactions; in pharmaceutical research for drug solubility, diffusion and cytosolic-targeting studies; in biomaterials testing to assess the biocompatibility of materials exposed to intracellular environments; and in molecular and synthetic biology for enzyme kinetics, biosensor calibration and reaction modelling under cytosol-like conditions.
It is supplied stabilized and has a 1-year shelf life unopened; a preservative-free version (6-month shelf life) and a dried Powder Version Kit are available on request. Tailored ionic profiles and additional biological additives are available on request. This simulated fluid is for scientific research and in vitro use only; it is not for clinical diagnosis or treatment. For the endosome see Artificial Endosomal Body Fluid (BZ260); for the fluid outside the cell see Simulated Extracellular Fluid (BZ280).
Key Features & Benefits
✅ Physiological Relevance – Ionic strength, pH and solute composition of natural cytosolic fluid
✅ pH 7.2 – Range 7.2 to 7.4; customizable
✅ High Purity & Consistency – Stringent quality control for batch-to-batch consistency
✅ Stabilized – Contains a preservative that stops microbial growth; 1-year shelf life unopened. Preservative-free version on request
✅ Sterile & Ready to Use – No weighing, mixing or pH adjustment before the test
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧫 Cell Biology & Biochemistry
• Cellular metabolism and signalling pathway studies
• Cytosolic protein interactions
💊 Pharmaceutical Research
• Drug solubility and diffusion
• Cytosolic-targeting studies
🧪 Biomaterials Testing
• Biocompatibility of materials exposed to intracellular environments
🔬 Molecular & Synthetic Biology
• Enzyme kinetics and reaction modelling
• Biosensor calibration under cytosol-like conditions
Typical Properties
- pH: 7.2 (range 7.2 to 7.4; customizable)
- Electrolytes: Sodium (Na⁺), potassium (K⁺), magnesium (Mg²⁺), calcium (Ca²⁺), chloride (Cl⁻), phosphate (HPO₄²⁻), sulfate (SO₄²⁻)
- Organic molecules: Glucose, amino acids, reducing agents (e.g. GSH); ATP precursors optional
- Buffer system: HEPES- or Tris-based
- Format: Sterile, ready-to-use liquid, stabilized
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, protected from light
- Transportation: At room temperature
- Shelf Life: 1 year (unopened)
- Preservative-Free Version: Available on request – shelf life 6 months unopened at 2–30 °C
- Powder Version Kit: Available on request – 2-year shelf life, easy to reconstitute and suitable for culture-related applications; recommended when a preservative is not required
- After Opening: Handle aseptically; close tightly after use and do not return unused material to the container
Customization Options
Customization available on request:
- pH adjustment to the project requirement
- Tailored ionic profiles
- Additional biological additives
- Preservative-free (not-stabilized) version
- Powder Version Kit, custom pack sizes and bulk carboys (5 L, 10 L, 20 L)
Frequently Asked Questions
What does BZ495 contain?
The electrolytes sodium, potassium, magnesium, calcium, chloride, phosphate and sulfate, with glucose, amino acids and reducing agents such as GSH, in a HEPES- or Tris-based buffer at pH 7.2. ATP precursors and other additives are optional.
How long does the solution keep?
Unopened, the stabilized solution has a 1-year shelf life at 2–30 °C. A preservative-free version with a 6-month shelf life and a dried Powder Version Kit with a 2-year shelf life are available on request.
Ordering Information
BZ495 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
