C. elegans M9 Buffer (Worm Wash and Handling Buffer) BZ718
Product Code: BZ718
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 500 mL, 1000 mL
Custom Bulk Quantities Available
Product Overview
Biochemazone C. elegans M9 Buffer BZ718 is the standard buffer for washing, transferring and synchronising Caenorhabditis elegans. It is used to wash worms off plates, in bleach synchronisation, for starvation arrest of L1 larvae and in behavioural and imaging assays.
M9 is a potassium and sodium phosphate buffer with sodium chloride and magnesium sulfate, at a pH of about 7.0 (range 6.8–7.2). It is supplied sterile, ready to use (1x), or as a 10x concentrate with the magnesium sulfate in a separate vial, since magnesium is added after dilution to avoid precipitation. It contains no animal-derived ingredients.
Note that worm M9 buffer is not the same as M9 minimal medium used for bacteria. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Standard Worm Buffer – Wash, transfer and synchronise
✅ pH About 7.0 – Range 6.8–7.2
✅ Sterile 1x – Ready to use
✅ 10x Concentrate – Magnesium supplied separately
✅ No Precipitate – Magnesium added after dilution
✅ Low Cost – For daily lab use
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🪱 Worm Handling
• Washing worms off plates
• Transfers and pick-free handling
• Removing bacteria before assays
🧬 Synchronisation
• Bleach (hypochlorite) synchronisation
• L1 arrest in buffer
🔬 Assays
• Behavioural and thrashing assays
• Imaging and drug-exposure assays
Usage Areas
- C. elegans laboratories
- Genetics and ageing research groups
- Drug-screening laboratories
- Universities
- Teaching laboratories
Typical Properties
- Appearance: Clear, colourless liquid
- pH: about 7.0 (range 6.8–7.2)
- Composition: Potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, magnesium sulfate
- Sterility: Sterile (1x); 10x concentrate filter-sterilised
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Dilute the 10x concentrate before adding the magnesium sulfate
Customization Options
- M9 with Tween 20 or Triton X-100
- S basal and S medium
- NGM salt packs
- Carboys for large facilities
Ordering Information
BZ718 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research use only. Not for veterinary or human therapeutic use.
