Gas-Test Buffer Medium Salt Kit (Hohenheim Gas Test, Menke Method) BZ681
Product Code: BZ681
Manufacturer: Biochemazone
Available Pack Sizes: Salt kits that make 1 L, 5 L or 10 L of medium
Custom Bulk Quantities Available
Product Overview
Biochemazone Gas-Test Buffer Medium Salt Kit BZ681 supplies the buffer and mineral components of the medium used in the Hohenheim gas test, the in-vitro gas production method of Menke et al. (1979) and Menke and Steingass (1988). Feed samples are incubated with rumen fluid and this buffered medium in glass syringes, and the gas produced is used to estimate digestibility and energy value.
The kit contains pre-weighed bicarbonate buffer salts, macro-mineral and micro-mineral salts and resazurin redox indicator, with instructions for making up the stock solutions. The reducing solution and the rumen fluid are not included and are prepared on site. After gassing with CO₂ at 39 °C, the complete medium has a pH of about 6.8–7.0. The kit contains no animal-derived ingredients.
Check the proportions and preparation in the edition of the method you follow. Supplied dry, the kits ship cheaply and keep for two years. Made under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Hohenheim Gas Test – Menke method buffer and minerals
✅ Pre-Weighed Salts – Buffer, macro- and micro-minerals
✅ Resazurin Included – Redox indicator for anaerobic set-up
✅ pH About 6.8–7.0 – After CO₂ gassing at 39 °C
✅ 24-Month Shelf Life – Stored dry
✅ Makes 1 L to 10 L – For single runs or large series
✅ No Animal Ingredients – Rumen fluid added on site
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🐄 Feed Evaluation
• Gas production and digestibility estimates
• Metabolisable energy of forages
• Feed-additive screening
🌿 Methane Research
• Screening of methane-reducing additives
• Fermentation kinetics
🎓 Education
• Animal-nutrition teaching labs
• Student research projects
Usage Areas
- Feed-analysis laboratories
- Animal-science departments
- Feed and additive companies
- Agricultural research institutes
- Contract research organisations
Typical Properties
- Appearance: White crystalline salts in separate packs; resazurin in a small vial
- pH (complete medium, CO₂-gassed): about 6.8–7.0
- Composition: Bicarbonate buffer salts, macro-mineral salts (phosphates, magnesium), micro-mineral salts and resazurin, per the Menke method
- Not included: Reducing solution and rumen fluid
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 2 years unopened (dry salt kit)
- Shipping: Room temperature
- Keep tightly closed and dry; make up solutions on the day of use or as the method directs
Customization Options
- Ready-made stock solutions
- Reducing-agent pack
- Other in-vitro rumen media
- Large kits for commercial feed labs
Ordering Information
BZ681 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research and product testing only. Not for veterinary or human therapeutic use.
