Simulated Lung Fluid with DPPC Surfactant BZ577
Product Code: BZ577
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Simulated Lung Fluid with DPPC Surfactant BZ577 is a Gamble’s-type simulated lung fluid that also contains dipalmitoylphosphatidylcholine (DPPC), the main phospholipid of human lung surfactant. The formulation follows the surfactant-containing lung fluid described in the review of simulated biological fluids by Marques, Loebenberg and Almukainzi (2011), in which 0.02% w/v DPPC is added to the electrolyte base.
The surfactant lowers surface tension and improves wetting of hydrophobic particles, so inhaled drugs and airborne particles dissolve in a way that is closer to the deep lung than in a plain salt solution. The fluid is buffered to pH 7.4 (range 7.2–7.6), the pH of the lung lining fluid, and prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
BZ577 is used for dissolution testing of dry-powder inhaler and nebulised formulations, for solubility of inhaled drug particles, and for particle-toxicology studies of dusts, fibres and nanomaterials. For a surfactant-free version see Gamble’s Solution BZ284 or Simulated Lung Fluid BZ179.
Key Features & Benefits
✅ Contains DPPC Surfactant – 0.02% w/v dipalmitoylphosphatidylcholine, the main lung-surfactant phospholipid
✅ pH 7.4 (Range 7.2–7.6) – Buffered to the pH of the lung lining fluid
✅ Gamble’s-Type Electrolyte Base – Physiological salts of the interstitial lung fluid
✅ Better Particle Wetting – Lower surface tension than surfactant-free lung fluids
✅ Published Basis – Follows the surfactant lung fluid in Marques et al. (2011)
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Ready-to-Use – No lipid dispersion step for the user
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
💊 Inhaled Drug Development
• Dissolution testing of dry-powder inhaler and metered-dose inhaler formulations
• Solubility of micronised and poorly soluble inhaled drugs
• Comparison of formulations and particle-engineering approaches
🧪 Particle & Nanomaterial Toxicology
• Dissolution and biodurability of dusts, fibres and nanomaterials
• Metal release from inhaled particles
• Occupational and environmental exposure studies
🔬 Research
• Surfactant–particle interaction studies
• Comparison with surfactant-free lung fluids (Gamble’s solution, artificial lysosomal fluid)
• Method development for in vitro lung models
Usage Areas
- Inhalation and respiratory pharmaceutical laboratories
- Particle-toxicology and occupational-health laboratories
- Nanomaterial safety researchers
- Contract research and testing organisations
- Universities and environmental-health institutes
Typical Properties
- Appearance: Clear to slightly opalescent liquid
- pH: 7.4 (range 7.2–7.6)
- Surfactant: DPPC 0.02% w/v (0.2 g/L)
- Electrolyte base: Gamble’s-type physiological salts
- Water Grade: ISO 3696 purified water
- Biological Status: Non-biological synthetic fluid
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Best kept at 2–8 °C for prolonged storage and stability of the DPPC surfactant components; storage at 2–30 °C is fine for short-term use
- Warm to 37 °C and invert gently before use; do not shake vigorously (foams)
Customization Options
- DPPC concentration adjustment
- Other surfactant phospholipids on request
- pH adjustment (for example pH 4.5 lysosomal version)
- Custom pack sizes and bulk / OEM supply
Ordering Information
BZ577 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
