INFOGEST 2.0 Simulated Digestion Fluid Set (SSF, SGF and SIF Electrolyte Stock Solutions) BZ576
Product Code: BZ576
Manufacturer: Biochemazone
Available Pack Sizes: Set of 3 bottles (SSF, SGF, SIF): 3 × 100 mL, 3 × 200 mL, 3 × 500 mL, 3 × 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone INFOGEST 2.0 Simulated Digestion Fluid Set BZ576 provides the three electrolyte stock solutions used in the INFOGEST static in vitro digestion method: simulated salivary fluid (SSF), simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). INFOGEST is the international consensus protocol for simulating food digestion, first published in 2014 and updated as INFOGEST 2.0 in Nature Protocols (2019).
The stocks are supplied as 1.25× concentrates, as the protocol describes, so that enzymes, bile, calcium chloride and water can be added at the time of use to reach the final working concentration. The pH is adjusted during the digestion to the protocol values: oral phase pH 7.0, gastric phase pH 3.0 and intestinal phase pH 7.0.
The set saves the weighing and preparation of six separate salt stock solutions for each fluid and gives every digestion run the same electrolyte background. Enzymes and bile are not included, because the protocol requires their activity to be measured and dosed by the user. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Made for the INFOGEST 2.0 Method – Electrolyte stocks for the oral, gastric and intestinal phases
✅ Three Matched Fluids – SSF, SGF and SIF supplied together as one set
✅ Protocol pH Values – Oral pH 7.0, gastric pH 3.0, intestinal pH 7.0 (adjusted during digestion)
✅ 1.25× Concentrates – Leaves volume for enzymes, bile, calcium chloride and water
✅ Enzyme-Free – Add your own characterised amylase, pepsin, pancreatin and bile
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Consistent Batch-to-Batch – Same electrolyte background in every digestion run
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧪 Food Digestion Studies
• Static in vitro digestion of foods and ingredients (INFOGEST 2.0)
• Protein digestibility and peptide release
• Lipid and starch digestion studies
🥗 Nutrition & Bioaccessibility
• Bioaccessibility of vitamins, minerals, polyphenols and carotenoids
• Release of bioactives from functional foods and supplements
• Comparison of food structures and processing methods
🔬 Product Development & Research
• Encapsulation and delivery-system testing
• Probiotic survival through the upper digestive tract
• Allergen and novel-protein digestion research
Usage Areas
- Food science and nutrition research laboratories
- Food, dairy and ingredient manufacturers
- Nutraceutical and supplement developers
- Alternative-protein and infant-nutrition developers
- Universities and contract research organisations
Typical Properties
- Appearance: Clear, colourless liquids
- pH in use: SSF 7.0; SGF 3.0; SIF 7.0 (adjusted during the digestion, as the protocol describes)
- Form: 1.25× electrolyte stock solutions
- Composition: Potassium, sodium, magnesium and ammonium salts (chloride, phosphate, bicarbonate) at the protocol concentrations; calcium chloride is added separately at the time of use
- Not included: Enzymes, bile, calcium chloride
- Water Grade: ISO 3696 purified water
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Warm to 37 °C before use; keep bottles closed, because bicarbonate-containing solutions lose carbon dioxide on standing
Customization Options
- Single fluids (SSF, SGF or SIF) supplied separately
- Calcium chloride solution supplied as a fourth bottle
- Working-strength (1×) fluids
- Custom pack sizes and bulk / OEM supply
Ordering Information
BZ576 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research and in vitro testing only. Not for human or animal use.
