Human Peripheral Simulated Blood Plasma – Chemically Defined, Protein-Enriched Plasma Simulant, pH 7.4 BZ464
Product Code: BZ464
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Human Peripheral Simulated Blood Plasma BZ464 is a sterile, ready-to-use, chemically defined, protein-enriched plasma simulant that mimics the electrolyte, metabolite and protein composition of normal adult human peripheral plasma. The pH is 7.35 to 7.45 and the osmolarity 280 to 300 mOsm/kg. It contains the electrolytes sodium, potassium, calcium, magnesium, chloride, phosphate and bicarbonate at physiological concentrations; human serum albumin and select globulins, with no animal-derived protein; glucose, lactate, urea, creatinine and key amino acids at fasting levels; and trace elements such as iron, zinc and copper, with buffering agents.
The formulation is benchmarked against reference ranges for healthy adult plasma. It has been validated in cell culture with PBMCs, macrophage activation assays and primary cell maintenance, and the preservative-free version is used in antifungal susceptibility studies and bacterial growth assays where native plasma conditions are wanted; validation reports can be shared on request. BZ464 is used for drug–plasma protein interaction and pharmacokinetic studies, biomaterial and implant compatibility, evaluation of medical devices such as stents and catheters, tissue engineering, dissolution testing, and corrosion and biodegradation studies of materials.
It is supplied stabilized and has a 1-year shelf life unopened; a preservative-free version (6-month shelf life) and a dried Powder Version Kit are available on request. Because it contains protein, keep it at 2–8 °C if it is to be stored for a long time. For cell culture and microbiology, order the preservative-free version or the Powder Version Kit, which is prepared on site with sterile water. Shake the bottle well before use. For assays that need closer biological mimicry, such as T-cell stimulation or antibody production, supplementing with 5–10% pooled human serum or heat-inactivated FBS is recommended. Each lot is supplied with a Certificate of Analysis. This simulated fluid is for scientific research and in vitro use only; it is not for clinical diagnosis or treatment. For the general plasma simulant see Artificial Plasma Fluid (BZ273); for a protein-free one see Protein Free Plasma Substitute (BZ437).
How BZ464 compares with a protein-free plasma-like medium (Gibco HPLM, A4899101)
| Feature | BZ464 – Human Peripheral Simulated Blood Plasma | Gibco HPLM |
|---|
| Protein source | Contains human serum albumin (recombinant/derived), mimicking the protein-bound fraction of plasma | Protein-free |
| Metabolites | Includes urea, creatinine, lactate and uric acid in the physiological range | Focused on essential nutrients; lacks waste metabolites |
| Buffer system | HCO₃⁻/CO₂ system with physiological buffering | HEPES/CO₂ |
| Application scope | Immune cell assays, microbial studies, biocompatibility of devices | Primarily cell culture that needs near-physiological metabolite support |
| Customization | Available on request | Fixed formulation |
Key Features & Benefits
✅ Chemically Defined – Electrolytes, metabolites and proteins of adult human peripheral plasma
✅ Protein-Enriched – Human serum albumin and select globulins; no animal-derived protein
✅ pH 7.35–7.45 – Osmolarity 280–300 mOsm/kg; bicarbonate/CO₂ buffering
✅ Validated – PBMCs, macrophage activation assays and primary cells; reports on request
✅ Stabilized – Contains a preservative that stops microbial growth; 1-year shelf life unopened. Preservative-free version on request
✅ Sterile & Ready to Use – No weighing, mixing or pH adjustment before the test
✅ Lot-Specific CoA – pH, osmolarity and protein content reported for every lot
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
💊 Pharmaceutical Research
• Drug interaction with plasma proteins
• Pharmacokinetics and drug delivery systems
• Dissolution testing in plasma-like conditions
🧫 Cell & Microbial Work
• PBMCs, macrophage activation assays, primary cells
• Antifungal susceptibility and bacterial growth assays (preservative-free version)
• Tissue engineering research
🩺 Devices & Biomaterials
• Biocompatibility of implants, scaffolds and coatings
• Stents and catheters in a simulated plasma environment
🧱 Materials Testing
• Corrosion of metal alloys and composites
• Degradation of biopolymers
• Bioavailability of nutrients and active ingredients
Typical Properties
- Appearance: Clear solution
- pH: 7.35 to 7.45 (standard 7.4; customizable)
- Osmolarity: 280–300 mOsm/kg
- Electrolytes: Sodium, potassium, calcium, magnesium, chloride, phosphate, bicarbonate
- Proteins: Human serum albumin and select globulins; no animal-derived protein
- Metabolites: Glucose, lactate, urea, creatinine and key amino acids at fasting levels
- Other: Trace elements (iron, zinc, copper) and buffering agents
- Format: Sterile, ready-to-use liquid, stabilized
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, protected from light
- Transportation: At room temperature
- Shelf Life: 1 year (unopened)
- Preservative-Free Version: Available on request – shelf life 6 months unopened at 2–30 °C
- Protein-Containing Product: Best kept at 2–8 °C for prolonged storage and stability; storage at 2–30 °C is fine for short-term use
- Powder Version Kit: Available on request – 2-year shelf life, easy to reconstitute and suitable for culture-related applications; recommended when a preservative is not required
- After Opening: Handle aseptically; close tightly after use and do not return unused material to the container
Customization Options
Customization available on request:
- pH adjustment (standard 7.4)
- Protein content and ionic strength
- Preservative-free (not-stabilized) version
- Powder Version Kit, custom pack sizes and bulk carboys (5 L, 10 L, 20 L)
Frequently Asked Questions
Which version should I order for cell culture or microbiology?
The preservative-free version, or the Powder Version Kit prepared on site with sterile water. The standard stabilized version contains a preservative and suits materials, device and analytical work.
Does BZ464 vary from batch to batch?
Each batch is made under ISO-certified conditions with validated consistency, and a Certificate of Analysis with pH, osmolarity and protein content is supplied for each lot.
How should it be stored?
Store it at 2–30 °C; because it contains protein, 2–8 °C is best for prolonged storage. It is shipped at room temperature. Unopened, the stabilized solution has a 1-year shelf life; a preservative-free version with a 6-month shelf life and a dried Powder Version Kit with a 2-year shelf life are available on request.
Ordering Information
BZ464 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
