FeSSGF (Fed State Simulated Gastric Fluid) – Biorelevant Fed-State Gastric Medium, pH 4.5 BZ415
Product Code: BZ415
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone FeSSGF (Fed State Simulated Gastric Fluid) BZ415 is a ready-to-use synthetic solution that mimics the properties of gastric fluid in the fed state, at pH 4.5. The pH of the stomach after a meal is about 3 to 6. FeSSGF contains water, electrolytes, enzymes and other digestive substances that replicate the physical and chemical properties of the natural fluid. Its viscosity, density and pH are set to match the natural fluid, and it stays stable through the test.
It is a biorelevant medium for in vitro studies of how oral dosage forms behave in the stomach after a meal. Research laboratories use it to study the interaction between gastric fluid and the fed-state environment, including digestive health and gastric function. It is also used to test and calibrate medical devices that work with gastric fluid, in the development of new technologies and procedures for the gastrointestinal tract, and in training, where it takes the place of human gastric fluid.
It is supplied stabilized and has a 1-year shelf life unopened; a preservative-free version (6-month shelf life) and a dried Powder Version Kit are available on request. Because it contains enzymes, keep it at 2–8 °C if it is to be stored for a long time. The formulation, minerals, organic content and pH can be customized. This simulated fluid is for scientific research and in vitro use only; it is not for clinical diagnosis or treatment. For the fed intestine see Fed State Simulated Intestinal Fluid (FeSSIF); for the early, middle and late fed stomach see Fed-State Gastric Media, Snapshot FeSSGF (BZ602).
Key Features & Benefits
✅ Fed-State Gastric Fluid – Water, electrolytes, enzymes and other digestive substances
✅ pH 4.5 – Within the fed-state range of about 3 to 6; customizable
✅ Matched Properties – Viscosity, density and pH close to the natural fluid
✅ Stabilized – Contains a preservative that stops microbial growth; 1-year shelf life unopened. Preservative-free version on request
✅ Ready to Use – No weighing, mixing or pH adjustment before the test
✅ Reproducible & Standardized – Consistent batch-to-batch composition for routine QC and inter-laboratory comparison
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
💊 Biorelevant Dissolution
• Behaviour of oral dosage forms in the stomach after a meal
• Fed-state gastric studies
🩺 Device Testing & Calibration
• Medical devices that work with gastric fluid
• Diagnostic tools for digestive health
🎓 Research & Training
• New technologies and procedures for the gastrointestinal tract
• Realistic scenarios without human gastric fluid
Typical Properties
- pH: 4.5 (fed-state range about 3 to 6; customizable)
- Composition: Water, electrolytes, enzymes and other digestive substances
- Viscosity and density: Matched to fed-state gastric fluid
- Format: Ready-to-use liquid, stabilized
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, protected from light
- Transportation: At room temperature
- Shelf Life: 1 year (unopened)
- Preservative-Free Version: Available on request – shelf life 6 months unopened at 2–30 °C
- Enzyme-Containing Product: Best kept at 2–8 °C for prolonged storage and activity; storage at 2–30 °C is fine for short-term use
- Powder Version Kit: Available on request – 2-year shelf life, easy to reconstitute and suitable for culture-related applications; recommended when a preservative is not required
- After Opening: Handle aseptically; close tightly after use and do not return unused material to the container
Customization Options
Customization available on request:
- pH adjustment to the project requirement
- Formulation, minerals and organic content
- Preservative-free (not-stabilized) version
- Powder Version Kit, custom pack sizes and bulk carboys (5 L, 10 L, 20 L)
Frequently Asked Questions
How does FeSSGF differ from simulated gastric fluid?
FeSSGF represents the stomach after a meal, at pH 4.5. Simulated Gastric Fluid (SGF) pH 1.5 (BZ175) represents the acid, fasting stomach at pH 1.5.
How should it be stored?
Store it at 2–30 °C; because it contains enzymes, 2–8 °C is best for prolonged storage and to keep the enzymes active. It is shipped at room temperature. Unopened, the stabilized solution has a 1-year shelf life; a preservative-free version with a 6-month shelf life and a dried Powder Version Kit with a 2-year shelf life are available on request.
Ordering Information
BZ415 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
