Simulated Body Fluid (SBF) ISO 23317 Method – Test Solution for the Apatite-Forming Ability of Implant Materials, pH 7.2–7.4 BZ361
Product Code: BZ361
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Simulated Body Fluid (SBF) ISO 23317 Method BZ361 is a sterile, ready-to-use SBF formulated in accordance with ISO 23317:2014 and ISO 23317:2025, the international method for evaluating the apatite-forming ability of implant materials in vitro. It replicates the ionic composition of human blood plasma (see the table below) and is supplied at pH 7.2 to 7.4. SBF is a metastable solution – a solution supersaturated with respect to apatite, containing calcium ions and phosphate ions. It is widely used for the in vitro evaluation of bioactive materials, on which it can form bone-like apatite.
When a bioactive material comes into contact with SBF, a calcium- and phosphorus-rich apatite layer forms on its surface, replicating the natural process of bone mineralization; it is this layer that lets an implant bond to living bone. BZ361 is used to test surgical implant surfaces for bioactivity and bone-integration potential in orthopaedic, dental and other surgical applications. It is also used to study drug release from injectable formulations and implantable drug delivery systems, and in the research and development of new biomaterials and pharmaceutical formulations.
Unopened, it has a 1-year shelf life at 2–30 °C and is shipped at room temperature; a preservative-free version and a dried Powder Version Kit are available on request. This reagent is for scientific research only. For the dry kit see Simulated Body Fluid (SBF) Dry Kit BZ585; for the concentrated solution see Simulated Body Fluid 10x (SBF) BZ472; for the other formulations see Simulated Body Fluid Corrected (c-SBF) (BZ310), Revised SBF (r-SBF) (BZ332) and Newly Improved SBF (n-SBF) (BZ333).
Ion concentrations (mM) of SBF and human blood plasma
| Ion | Simulated body fluid | Blood plasma |
|---|
| Na⁺ | 142.0 | 142.0 |
| K⁺ | 5.0 | 5.0 |
| Mg²⁺ | 1.5 | 1.5 |
| Ca²⁺ | 2.5 | 2.5 |
| Cl⁻ | 148.8 | 103.0 |
| HCO₃⁻ | 4.2 | 27.0 |
| HPO₄²⁻ | 1.0 | 1.0 |
| SO₄²⁻ | 0.5 | 0.5 |
Key Features & Benefits
✅ ISO 23317 Method – Formulated in accordance with ISO 23317:2014 and ISO 23317:2025
✅ Human Plasma Analogue – Matches the ionic composition of blood plasma
✅ Apatite-Forming Evaluation – For the bioactivity and bonding potential of implant surfaces
✅ 1-Year Shelf Life – Unopened at 2–30 °C; shipped at room temperature
✅ Sterile & Ready to Use – No weighing, mixing or pH adjustment before the test
✅ Reproducible & Standardized – Consistent batch-to-batch composition for routine QC and inter-laboratory comparison
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🦴 Implant Bioactivity (ISO 23317)
• Apatite-forming ability of surgical implant surfaces
• Bone-integration potential of orthopaedic and dental materials
💊 Drug Release Studies
• Release rates from intramuscular, subcutaneous and intravenous delivery systems
• Injectable formulations and implantable drug delivery systems
🔬 Research & Development
• New biomaterials
• Pharmaceutical formulations
Typical Properties
- Appearance: Clear solution
- pH: 7.2–7.4
- Ionic composition: Na⁺ 142.0, K⁺ 5.0, Mg²⁺ 1.5, Ca²⁺ 2.5, Cl⁻ 148.8, HCO₃⁻ 4.2, HPO₄²⁻ 1.0, SO₄²⁻ 0.5 mM
- Prepared as per: ISO 23317:2014 and ISO 23317:2025
- Format: Sterile, ready-to-use liquid
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, protected from light
- Transportation: At room temperature
- Shelf Life: 1 year (unopened)
- Preservative-Free Version: Available on request
- Powder Version Kit: Available on request – 2-year shelf life, easy to reconstitute and suitable for culture-related applications
- After Opening: Handle aseptically; close tightly after use and do not return unused material to the container
Customization Options
Customization available on request:
- Contents to your specification
- Powder Version Kit, custom pack sizes and bulk carboys (5 L, 10 L, 20 L)
Frequently Asked Questions
What does ISO 23317 test?
The in vitro apatite-forming ability of implant materials: the material is soaked in SBF and its surface is examined for a layer of bone-like apatite.
How long does the solution keep?
Unopened, the solution has a 1-year shelf life at 2–30 °C. A preservative-free version and a dried Powder Version Kit with a 2-year shelf life are available on request.
Ordering Information
BZ361 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
