HBSS 1X (Hank’s Balanced Salt Solution), Sterile (BZ261)

Hank’s Balanced Salt Solution (HBSS) 1X, Sterile BZ261

Product Code: BZ261
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone Hank’s Balanced Salt Solution (HBSS) BZ261 is a sterile, isotonic balanced salt solution that keeps cells and tissues at physiological pH and osmolality outside the incubator. It supplies the inorganic ions and glucose that cells need for short-term maintenance, making it the standard solution for cell washing, tissue dissection and transport, and short-term incubations.

HBSS uses a low-bicarbonate phosphate buffer system designed for room air, so it holds pH 7.0–7.4 on the bench without a CO₂ incubator. The standard formulation contains calcium and magnesium; calcium- and magnesium-free, phenol red and HEPES-buffered versions are made on request.

Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ Balanced Salt Solution – Physiological ions plus glucose
✅ Works in Room Air – Low-bicarbonate buffer for use outside a CO₂ incubator
✅ pH 7.0–7.4 – Physiological pH range
✅ Isotonic – Maintains cell volume and viability
✅ Sterile – Ready to use for aseptic work
✅ Flexible Formulation – With or without Ca/Mg, phenol red or HEPES on request
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🧫 Cell Culture
• Washing and rinsing cells
• Holding cells for short periods outside the incubator
• Diluent for cells and reagents

🧠 Tissue Work
• Tissue dissection and transport
• Enzymatic tissue dissociation (calcium/magnesium-free version)
• Primary cell isolation

🔬 Assays
• Buffer for uptake, transport and calcium-imaging assays
• Base for staining solutions

Usage Areas

• Cell and tissue culture laboratories
• Neuroscience and physiology laboratories
• Primary cell isolation units
• Pharmacology and drug-screening groups
• Academic laboratories

Composition (standard formulation, per litre)

Standard formulation with calcium and magnesium, without phenol red. Calcium/magnesium-free and phenol red versions are made on request.

Typical Properties

• Appearance: Clear, colourless liquid
• pH: 7.0–7.4 in room air
• Concentration: 1X
• Osmolality: Isotonic (typically about 270–300 mOsm/kg)
• Glucose: 1 g/L
• Sterility: Sterile

Available Formulations

• HBSS with calcium and magnesium (standard, BZ261)
• HBSS without calcium and magnesium (on request)
• HBSS with phenol red or with 10–25 mM HEPES (on request)
• Earle’s Balanced Salt Solution for CO₂ incubators (BZ232)
• DPBS without calcium and magnesium (BZ221)

Storage, Shelf Life & Transportation

• Storage Temperature: 2–30 °C
• Shelf Life: 1 year for unopened bottles
• Transportation: Room temperature (ambient shipping)
• Version with Preservatives: Available on request
• Powder Version: Available on request
• Keep tightly closed to limit CO₂ loss and pH drift
• Use aseptic technique after opening; do not use if the solution turns cloudy

Customization Options

• Calcium- and magnesium-free
• With phenol red
• With HEPES (10–25 mM)
• 10X concentrate or bulk containers

Ordering Information

BZ261 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.