Earle’s Balanced Salt Solution (EBSS) 1X with Calcium, Magnesium & Phenol Red BZ232
Product Code: BZ232
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Earle’s Balanced Salt Solution (EBSS) BZ232 is an isotonic balanced salt solution with calcium, magnesium, phosphate, glucose and a high level of sodium bicarbonate. It keeps cells hydrated, supplies essential ions and holds physiological pH in a 5% CO2 atmosphere.
EBSS contains no amino acids, vitamins or serum. That makes it the classic medium for amino-acid and serum starvation – for example to induce autophagy – as well as for washing and rinsing cells, short-term holding of cells and tissue, and as the salt base for enzyme dissociation solutions. Phenol red gives a quick visual check of pH; because it is a weak oestrogen mimic, a phenol-red-free version is available for hormone-sensitive cells.
Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Classic Earle Formulation – Na+, K+, Ca2+, Mg2+, Cl−, SO42−, PO4
✅ With Glucose – 1 g/L energy source
✅ 26 mM Bicarbonate – For use in 5% CO2
✅ Amino-Acid-Free – Ideal for starvation and autophagy studies
✅ With Phenol Red – Visual pH indicator
✅ 100 mL to 1 L – Plus custom bulk volumes
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧫 Cell Culture
• Washing and rinsing adherent and suspension cells
• Short-term holding of cells in a CO2 incubator
• Diluent and salt base for dissociation enzymes
🧬 Cell Biology Research
• Amino-acid / serum starvation to induce autophagy
• Nutrient-deprivation and metabolic studies
🔬 Tissue Work
• Rinsing and holding freshly dissected tissue
• Oocyte, embryo and primary-cell handling (per protocol)
Usage Areas
- Cell culture and cell biology laboratories
- Autophagy and metabolism research groups
- Tissue engineering and primary-cell laboratories
- Biotechnology and pharmaceutical R&D
- Academic research laboratories
Composition (per litre)
| Component | Concentration | Amount per litre |
|---|
| Sodium chloride (NaCl) | 116 mM | 6.80 g |
| Potassium chloride (KCl) | 5.3 mM | 0.40 g |
| Calcium chloride (CaCl2) | 1.8 mM | 0.20 g |
| Magnesium sulfate (MgSO4) | 0.81 mM | 0.098 g |
| Sodium dihydrogen phosphate (NaH2PO4) | 1.0 mM | 0.122 g |
| Sodium bicarbonate (NaHCO3) | 26.2 mM | 2.20 g |
| D-Glucose | 5.6 mM | 1.00 g |
| Phenol red | pH indicator | trace |
| High-purity water (ISO 3696) | — | to 1 L |
Classic Earle formulation (anhydrous salt weights). pH about 7.2–7.4 when equilibrated with 5% CO2.
Typical Properties
- Appearance: Clear, red-orange liquid (phenol red)
- pH: About 7.2–7.4 in 5% CO2 (rises in room air)
- Osmolality: Isotonic (about 280–300 mOsm/kg)
- pH indicator: Phenol red
Available Formulations
- Earle’s balanced salt solution (EBSS) 1X (this product, BZ232)
- HBSS 1X (Hank’s balanced salt solution) (BZ261)
- DPBS 1X without Ca/Mg (BZ221)
- Tyrode’s solution (BZ212)
- 1X PBS pH 7.4, sterile (BZ205)
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Transportation: Room temperature (ambient shipping)
- Version with Preservatives: Available on request
- Powder Version: Available on request
- Keep tightly closed – bicarbonate solutions lose CO2 and the pH rises when left open
- After opening, handle aseptically
Customization Options
- Without calcium and magnesium
- Phenol-red-free version
- Without glucose or with HEPES
- Sterile-filtered, 10X concentrate or bulk containers
Ordering Information
BZ232 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com
⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.
