Description
Product overview
20X SSC Buffer (BZ231) is a saline-sodium citrate concentrate (3 M NaCl, 0.3 M sodium citrate, pH 7.0) for hybridization, blotting transfer and stringency washes.
- Catalogue no.: BZ231
- Concentration: 20X (3 M NaCl, 0.3 M sodium citrate)
- pH: 7.0
- 1X: 150 mM NaCl, 15 mM sodium citrate
- Pack sizes: 100 mL, 200 mL, 500 mL, 1 L
- Storage: 2–30 °C
- Shelf life: 1 year (unopened bottle)
- On request: Version with preservatives; powder version
- Transportation: Room temperature
- Use: Laboratory research use only
Frequently asked questions
How do I make 2X SSC from 20X SSC?
Mix 1 part BZ231 with 9 parts high-purity water. For 0.5X SSC use 1 part with 39 parts water, and for 0.1X SSC use 1 part with 199 parts water.
What is SSC used for?
SSC is the hybridization and transfer buffer in nucleic acid blotting and, at decreasing concentrations, the stringency-wash buffer in Southern, Northern, in situ hybridization and FISH protocols.
How does SSC concentration affect stringency?
Lower salt reduces the stability of mismatched hybrids, so washes in 0.5X or 0.1X SSC (often with 0.1% SDS) are more stringent than washes in 2X SSC.
Can I get SSC at another pH?
Yes. The standard product is pH 7.0; 20X SSC at pH 7.4 or pH 5.3 can be made on request.
How should 20X SSC be stored?
Store at 2–30 °C, tightly closed. Unopened bottles have a 1-year shelf life and ship at room temperature.
20X SSC Buffer
20X SSC Buffer composition and customer’s specified configurations can be delivered upon request. pH Value, Concentration, SDS Addition, Container Size, and Contents of the buffer are customizable.
Contact us for Customization: info@biochemazone.com
Transportation and storage: Transportation at room temperature, storage at 2–30 ℃
We provide shipping facilities worldwide.
