Artificial Intestinal Fluid (Sterile) – Simulated Intestinal Fluid (SIF) with Enzymes, pH 6.8 BZ176
Product Code: BZ176
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone Artificial Intestinal Fluid BZ176, also called Simulated Intestinal Fluid (SIF) or synthetic intestinal fluid, is a sterile, ready-to-use solution that simulates the components and pH of intestinal fluid, at pH 6.8 ± 0.1. It is mainly composed of phosphate with the enzymes trypsin and pancreatin, and is sterilized by 0.2 µm filtration.
It is used in scientific research experiments on capsules, enteric-coated tablets and pills, especially enteric-coated capsules. The standard product contains enzymes; a formulation without enzyme, or with other enzymes, is made on request.
It is supplied stabilized and has a 1-year shelf life unopened; a preservative-free version (6-month shelf life) and a dried Powder Version Kit are available on request. Because it contains enzymes, keep it at 2–8 °C if it is to be stored for a long time. The composition, pH, viscosity and enzymes can be customized. This simulated fluid is for scientific research and in vitro use only; it is not for clinical diagnosis or treatment. For the pharmacopoeial fluid see USP Simulated Intestinal Fluid TS (BZ575); for the biorelevant media see Fasted State Simulated Intestinal Fluid (FaSSIF) and Fed State Simulated Intestinal Fluid (FeSSIF).
Key Features & Benefits
✅ Simulates Intestinal Fluid – Phosphate with trypsin and pancreatin
✅ pH 6.8 ± 0.1 – Customizable to the project requirement
✅ With Enzymes – Enzyme-free formulation on request
✅ Stabilized – Contains a preservative that stops microbial growth; 1-year shelf life unopened. Preservative-free version on request
✅ Sterile-Filtered & Ready to Use – Sterilized by 0.2 µm filtration; no preparation before the test
✅ Reproducible & Standardized – Consistent batch-to-batch composition for routine QC and inter-laboratory comparison
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
💊 Dosage Form Research
• Enteric-coated capsules
• Capsules, enteric-coated tablets and pills
🔬 In Vitro Research
• Dissolution and release in an intestinal medium
• Comparison between batches and laboratories
Typical Properties
- Appearance: Clear to hazy with stringy fibres, colourless, characteristic odour
- pH: 6.8 ± 0.1 at 25 °C (customizable)
- Composition: Mainly phosphate, with trypsin and pancreatin
- Sterility: Sterilized by 0.2 µm filtration
- Format: Sterile, ready-to-use liquid, stabilized
Storage, Shelf Life & Transportation
- Storage Temperature: 2–30 °C, protected from light
- Transportation: At room temperature
- Shelf Life: 1 year (unopened)
- Preservative-Free Version: Available on request – shelf life 6 months unopened at 2–30 °C
- Enzyme Versions: Best kept at 2–8 °C for prolonged storage and activity; storage at 2–30 °C is fine for short-term use
- Powder Version Kit: Available on request – 2-year shelf life, easy to reconstitute and suitable for culture-related applications; recommended when a preservative is not required
- After Opening: Handle aseptically; close tightly after use and do not return unused material to the container
Customization Options
Customization available on request:
- pH adjustment to the project requirement
- Enzyme-free formulation or other enzymes
- Composition and viscosity
- Preservative-free (not-stabilized) version
- Powder Version Kit, custom pack sizes and bulk carboys (5 L, 10 L, 20 L)
Frequently Asked Questions
Does BZ176 contain enzymes?
Yes. The standard product contains trypsin and pancreatin. Tell us before dispatch if you need the formulation without enzyme.
How should it be stored?
Store it at 2–30 °C; because it contains enzymes, 2–8 °C is best for prolonged storage and to keep the enzymes active. It is shipped at room temperature. Unopened, the stabilized solution has a 1-year shelf life; a preservative-free version with a 6-month shelf life and a dried Powder Version Kit with a 2-year shelf life are available on request.
Ordering Information
BZ176 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
