Synthetic Cystic Fibrosis Sputum Medium – SCFM1 and SCFM2 BZ578
Product Code: BZ578
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L (SCFM1 or SCFM2) (premixed ready-to-use or Powder Version Kit)
Custom Bulk Quantities Available
Product Overview
Biochemazone Synthetic Cystic Fibrosis Sputum Medium BZ578 reproduces the nutrient environment of sputum from people with cystic fibrosis, for growing Pseudomonas aeruginosa and other CF pathogens under realistic conditions. Two published formulations are offered.
SCFM1 is the defined medium of Palmer, Aye and Whiteley (2007): amino acids, glucose, lactate and salts at the levels measured in CF sputum, at pH 6.8. SCFM2, described by Turner et al. (2015), adds the viscous components of sputum: mucin (5 g/L), DNA (0.6 g/L), the lipid DOPC (0.1 g/L) and N-acetylglucosamine (0.3 mM). In SCFM2, P. aeruginosa gene essentiality closely matches that seen in real CF sputum.
Both media are prepared aseptically for microbiology use with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system. For simpler mucin-based sputum media see Artificial Sputum Medium BZ316.
Two formats are offered: the premixed ready-to-use medium (shelf life 1 month at 2–30 °C, unopened) and a Powder Version Kit that is reconstituted easily with the instructions provided. For a 1-year shelf life we recommend the Powder Version Kit.
Key Features & Benefits
✅ Premixed or Powder Kit – Ready-to-use, or a 1-year powder kit with reconstitution instructions
✅ Two Published Formulations – SCFM1 (Palmer et al. 2007) and SCFM2 (Turner et al. 2015)
✅ pH 6.8 – Matches the pH of CF sputum
✅ Defined Nutrients – Amino acids, glucose and lactate at CF-sputum levels
✅ Sputum Polymers in SCFM2 – Mucin, DNA, DOPC lipid and N-acetylglucosamine
✅ Realistic Pathogen Behaviour – Supports CF-like growth, biofilm and gene expression
✅ Prepared for Microbiology – Aseptic preparation, ready to inoculate
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🦠 Cystic Fibrosis Infection Models
• Growth of P. aeruginosa, Burkholderia, S. aureus and other CF pathogens
• Biofilm and aggregate formation in sputum-like conditions
• Transcriptomics, Tn-seq and metabolomics under CF conditions
💊 Antimicrobial Testing
• Antibiotic susceptibility and tolerance in sputum-like media
• Phage therapy and antimicrobial-peptide testing
• Inhaled antibiotic and mucolytic evaluation
🔬 Research
• Polymicrobial and host-pathogen interaction studies
• Comparison with standard laboratory media
• Teaching of CF microbiology
Usage Areas
- Cystic fibrosis and respiratory infection research laboratories
- Antimicrobial-resistance research groups
- Pharmaceutical and biotech anti-infective developers
- Clinical microbiology research units
- Universities and teaching hospitals
Typical Properties
- Appearance: SCFM1 clear, colourless; SCFM2 viscous, opalescent
- pH: 6.8
- SCFM1: Defined amino acids, glucose, L-lactate and salts at CF-sputum concentrations
- SCFM2: SCFM1 plus mucin 5 g/L, DNA 0.6 g/L, DOPC 0.1 g/L and N-acetylglucosamine 0.3 mM
- Water Grade: ISO 3696 purified water
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life (premixed ready-to-use): 1 month at 2–30 °C, unopened; once opened, use within 3 days
- Shelf Life (Powder Version Kit): 1 year unopened
- Shipping: Room temperature
- Mix gently before use
- An antibiotic/preservative can be added on request to extend the shelf life of the premixed version
Customization Options
- Individual component adjustment (for example iron, oxygen-sensitive or amino-acid drop-out versions)
- Mucin source and concentration
- Optional antibiotic/preservative to extend the shelf life of the premixed version
- Custom pack sizes and bulk / OEM supply
Ordering Information
BZ578 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research use only. Not for human or animal use.
