Pig In-Vitro Digestion Fluids (Stomach and Small Intestine) BZ683
Product Code: BZ683
Manufacturer: Biochemazone
Available Pack Sizes: 500 mL, 1 L, 5 L (sets contain one of each fluid)
Custom Bulk Quantities Available
Product Overview
Biochemazone Pig In-Vitro Digestion Fluids BZ683 supply the stomach and small-intestine steps used to estimate the digestibility of pig feeds in the laboratory. They follow the multi-step approach of Boisen and Fernández (1997), in which feed is incubated first with pepsin at low pH and then with pancreatin at near-neutral pH, and the undigested residue is measured.
Two fluids are offered, singly or as a set. The stomach step is a phosphate buffer acidified to a pH of about 2.0, with pepsin. The small-intestine step is a phosphate buffer at a pH of about 6.8, with pancreatin. Pepsin and pancreatin are of porcine origin and are animal-derived; they are supplied as pre-weighed powders to add on the day of use. The antimicrobial and the large-intestine enzyme step used in the full method are not included.
Check incubation times, enzyme amounts and pH in the published method or your in-house protocol. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Pig Digestion Steps – Stomach and small intestine
✅ Boisen & Fernández Approach – Established in-vitro pig method
✅ Stated pH – Stomach 2.0; small intestine 6.8
✅ Enzymes Added Fresh – Pre-weighed pepsin and pancreatin
✅ Singles or Sets – One step or both
✅ Up to 5 L – For large sample series
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🐖 Feed Digestibility
• In-vitro protein and dry-matter digestibility
• Feed ingredient ranking
• Processing-effect studies
🧪 Enzymes & Probiotics
• Feed-enzyme efficacy screening
• Probiotic survival through the gut
• Phytase and protease evaluation
🎓 Research
• Animal-nutrition research
• Teaching laboratories
Usage Areas
- Feed manufacturers
- Feed-enzyme and probiotic companies
- Feed-analysis laboratories
- Animal-science departments
- Contract research organisations
Typical Properties
- Appearance: Clear, colourless liquids; enzymes as white to cream powders
- pH: stomach step about 2.0; small-intestine step about 6.8
- Composition: Phosphate buffers with pepsin (stomach) or pancreatin (small intestine); enzymes of porcine origin (animal-derived)
- Enzymes: Supplied separately as pre-weighed powders
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Best kept at 2–8 °C for prolonged storage and stability of the pepsin and pancreatin enzyme components; storage at 2–30 °C is fine for short-term use
- Add enzymes on the day of use; warm to 39 °C before incubation
Customization Options
- Large-intestine (fibre-degrading enzyme) step
- Antimicrobial added as your method requires
- Enzyme amounts matched to your protocol
- Bulk supply
Ordering Information
BZ683 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research and product testing only. Not for veterinary or human therapeutic use.
