Human Peripheral Simulated Blood Plasma BZ464

Human Peripheral Simulated Blood Plasma (BZ464)

Biochemazone™’s Human Peripheral Simulated Blood Plasma (BZ464) is a meticulously designed solution replicating the properties of natural peripheral blood plasma. Tailored for scientific and industrial applications, it serves as an essential medium for evaluating the interaction of various materials, compounds, and devices under physiologically relevant conditions.

This versatile testing fluid is indispensable for pharmaceutical, biomedical, and material sciences, enabling accurate studies of drug interactions, biomaterial compatibility, and tissue engineering. Whether exploring drug efficacy or analyzing implant behavior in plasma-like environments, BZ464 ensures reliable and reproducible results.

With customizable pH levels and formulation options, BZ464 meets diverse research requirements, guaranteeing precision and performance across scientific investigations.

Product Overview

• Formulation: Ready-to-use sterile liquid with physiologically relevant pH and ionic strength.
• Pack Sizes: Available in 100 mL, 200 mL, 500 mL, and 1000 mL.
• Stability:
  • Non-Stabilized/without Preservatives Version: Valid for up to 6 months at -20°C.
  • Stabilized Version: Valid for up to 12 months under refrigerated conditions.
  • Frozen Storage: Shelf life extended to 1 year at -20°C.
• Transportation and Storage: Shipped at room temperature; storage at 2-8°C for liquid form upon receiving. It can be stored at -20°C using small aliquots.

Key Features

• Simulated Composition: Closely mimics the mineral composition, proteins, and pH of natural peripheral blood plasma.
• Customizable: Adjustable pH, protein content, and ionic strength tailored to specific testing protocols.
• Versatile Application Range: Suitable for research across pharmaceutical, biomedical, and material science disciplines.
• Ease of Use: Pre-formulated and sterile, eliminating the need for preparation.

Applications of Human Peripheral Simulated Blood Plasma (BZ464):

1. Drug Interaction Studies:
   • Assess the interaction of pharmaceuticals with plasma proteins and evaluate pharmacokinetics and drug delivery systems.
2. Biomaterial Compatibility:
   • Test the biocompatibility of implants, scaffolds, and coatings in plasma-like conditions for tissue engineering and regenerative medicine.
3. Medical Device Evaluation:
   • Examine the stability and behavior of medical devices, such as stents and catheters, in a simulated plasma environment.
4. Tissue Engineering Research:
   • Support the growth and differentiation of cells in biomimetic environments for in vitro studies.
5. Nutritional Bioavailability Studies:
   • Investigate the bioavailability of nutrients and active ingredients in food and supplement formulations when exposed to plasma-like conditions.
6. Material Testing and Corrosion Studies:
   • Evaluate the degradation and corrosion of materials, such as metal alloys and composites, in plasma-like settings.
7. Biodegradability Analysis:
   • Study the degradation of biopolymers and other materials in simulated physiological environments.
8. Pharmaceutical Dissolution Testing:
   • Optimize drug formulations for controlled release and absorption by studying their dissolution behavior in plasma-mimicking conditions.

Customization Options:

• pH adjustments (standard: 7.4) and ingredient modifications to suit specific research needs.
• Powder format available for researchers preferring on-site preparation with sterile water.

1. Composition Details

BZ464 is a chemically defined, protein-enriched simulated plasma formulation designed to closely mimic the electrolyte, metabolite, and protein composition of normal adult human peripheral plasma. The formulation includes:

• Electrolytes: Sodium, potassium, calcium, magnesium, chloride, phosphate, and bicarbonate in physiologically relevant concentrations.
• Proteins: Human serum albumin (HSA) and select globulins. No animal-derived proteins are used.
• Metabolites: Glucose, lactate, urea, creatinine, and key amino acids present in human plasma at fasting physiological levels.
• pH and Osmolarity: Maintained between pH 7.35–7.45 and 280–300 mOsm/kg to emulate native plasma properties.
• Other components: Includes trace elements (e.g., iron, zinc, copper), and buffering agents to stabilize pH under typical culture conditions.

2. Validation Data

• Physiological Comparisons: The formulation has been benchmarked against reference ranges reported for healthy adult human plasma (e.g., WHO, NIH Clinical Chemistry Panel) to ensure accurate metabolite and electrolyte levels.
• Cellular and Microbial Validation:
  • Cell Culture: BZ464 has been validated for use in PBMCs, macrophage activation assays, and primary cell maintenance. Results showed better physiological response profiles compared to traditional basal media (e.g., RPMI or DMEM).
  • Microbiology: BZ464 without preservatives version used in antifungal susceptibility studies and bacterial growth assays where native plasma conditions are desired. Reduced artificial nutrient bias was observed.

Detailed validation reports can be shared upon request.

3. Comparison to Gibco HPLM (A4899101)

Feature	BZ464 – Human Peripheral Simulated Plasma	Gibco HPLM
Protein Source	Contains human serum albumin (recombinant/derived), mimicking protein-bound fraction of plasma	Protein-free
Metabolites	Includes urea, creatinine, lactate, uric acid in physiological range	Focused on essential nutrients, lacks waste metabolites
Buffer System	HCO₃⁻/CO₂ system with physiologic buffering	HEPES/CO₂
Application Scope	Immune cell assays, microbial studies, biocompatibility of devices	Primarily for cell culture requiring near-physiologic metabolite support
Customization	Available upon request	Fixed formulation

4. Batch-to-Batch Variability

We maintain strict quality control protocols, and each batch of BZ464 is manufactured under ISO-certified conditions with validated consistency. Certificate of Analysis (CoA) is provided for each lot, detailing key physicochemical parameters (e.g., pH, osmolarity, protein content).

5. Supplementation Recommendations

For applications requiring enhanced biological mimicry—such as T-cell stimulation assays or antibody production—we recommend supplementing BZ464 with 5–10% pooled human serum or heat-inactivated fetal bovine serum (FBS), depending on assay sensitivity and ethical considerations.

Recommendations for use: The solution must be shaken thoroughly prior to use. The shelf life of this product is guaranteed until the expiry date, provided the bottle is unopened and stored at ambient temperature (2 °C to 8 °C).

Order Biochemazone™’s Human Peripheral Simulated Blood Plasma (BZ464) for precise and reliable results across your research and development applications.