FaSSIF-V2 (Fasted State Simulated Intestinal Fluid, Version 2) BZ572
Product Code: BZ572
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available
Product Overview
Biochemazone FaSSIF-V2 BZ572 is the updated fasted-state simulated intestinal fluid described by Jantratid et al. (2008). Compared with the original FaSSIF, Version 2 uses a lower lecithin level and a maleate buffer in place of phosphate, which brings its solubilising capacity and osmolality closer to fluid aspirated from the fasted human small intestine.
The medium contains bile salt (sodium taurocholate, 3 mM) and lecithin (0.2 mM) in a maleate buffer at pH 6.5, with an osmolality of about 180 mOsm/kg. It is supplied ready to use, prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
BZ572 is used for biorelevant dissolution and solubility testing of poorly soluble drugs, for formulation screening, and for studying how a product is likely to behave in the fasted small intestine before in vivo work.
Key Features & Benefits
✅ Version 2 Composition – Follows the updated fasted-state medium published in 2008
✅ pH 6.5 – Maleate-buffered to the fasted small-intestine pH
✅ Bile Salt + Lecithin – Sodium taurocholate 3 mM and lecithin 0.2 mM form biorelevant mixed micelles
✅ Physiological Osmolality – About 180 mOsm/kg
✅ Enzyme-Free – No pancreatin; suitable for dissolution and solubility work
✅ Ready-to-Use – No powder weighing or equilibration step
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧪 Biorelevant Dissolution Testing
• Dissolution profiles of immediate- and modified-release products
• Two-stage gastric-to-intestinal transfer experiments
• Comparison of formulations and batches
💊 Solubility & Formulation Screening
• Equilibrium and kinetic solubility of poorly soluble drugs
• Precipitation and supersaturation studies
• Selection of enabling formulations (amorphous dispersions, lipid systems)
🔬 Biopharmaceutics Research
• Input data for absorption modelling (PBPK)
• Fasted versus fed comparison with FeSSIF-V2
• In vitro–in vivo correlation studies
Usage Areas
- Pharmaceutical formulation and pre-formulation laboratories
- Analytical development and dissolution laboratories
- Contract research and development organisations
- Generic-drug developers
- University pharmaceutics departments
Typical Properties
- Appearance: Clear to slightly opalescent liquid
- pH: 6.5
- Bile Salt: Sodium taurocholate, 3 mM
- Lecithin: 0.2 mM
- Buffer: Maleate
- Osmolality: about 180 mOsm/kg
- Water Grade: ISO 3696 purified water
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Best kept at 2–8 °C for prolonged storage and stability of the bile salt and lecithin components; storage at 2–30 °C is fine for short-term use
- Warm to 37 °C and mix gently before use; use opened medium within 48 hours
Customization Options
- pH and buffer adjustment
- Bile salt and lecithin level adjustment
- Addition of pancreatin on request
- Custom pack sizes and bulk / OEM supply
Ordering Information
BZ572 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research, education, and in vitro testing only. Not for human or animal use.
