Pet-Food In-Vitro Digestibility Fluids (Pepsin and Pancreatin Phases) BZ706
Product Code: BZ706
Manufacturer: Biochemazone
Available Pack Sizes: Kits that make 1 L or 5 L of each phase
Custom Bulk Quantities Available
Product Overview
Biochemazone Pet-Food In-Vitro Digestibility Fluids BZ706 are a two-phase set for estimating the digestibility of dog and cat foods in the laboratory. They follow the general approach of the pepsin–pancreatin method described by Hervera et al. (2007) for extruded dog foods, in which the food is first incubated in an acidic pepsin phase and then in a near-neutral pancreatin phase, and the undigested residue is measured.
The kit contains a gastric buffer at pH 2.0 with a separate vial of pepsin, and an intestinal phosphate buffer at pH 6.8 with a separate vial of pancreatin. The enzymes are supplied dry and added on the day of use, so their activity is not lost in storage. Both enzymes are animal-derived (porcine). Enzyme activity is reported for each lot.
Incubation times, temperature and sample weights should follow your validated method. Check the details against the published method you are following. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Two-Phase Method – Pepsin phase, then pancreatin phase
✅ Gastric pH 2.0 – Intestinal phase pH 6.8
✅ Dry Enzyme Vials – Added fresh; no loss of activity in storage
✅ Activity Reported – Pepsin and pancreatin activity per lot
✅ Dog and Cat Foods – Extruded, canned and raw diets
✅ Kit Sizes – Makes 1 L or 5 L of each phase
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🐕 Pet-Food Development
• Screening new recipes and ingredients
• Comparing processing conditions
• Protein-source evaluation
🧪 Quality Control
• Batch-to-batch digestibility checks
• Supplier ingredient screening
🎓 Research
• Companion-animal nutrition studies
• Reducing the number of feeding trials
Usage Areas
- Pet-food manufacturers
- Ingredient suppliers
- Animal nutrition laboratories
- Veterinary and animal science departments
- Contract research organisations
Typical Properties
- Appearance: Clear, colourless buffers; enzymes as off-white powders
- pH: gastric buffer 2.0 ± 0.1; intestinal buffer 6.8 ± 0.1
- Composition: Hydrochloric acid–salt gastric buffer with porcine pepsin; phosphate intestinal buffer with porcine pancreatin
- Animal-derived components: Pepsin and pancreatin (porcine)
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Best kept at 2–8 °C for prolonged storage and stability of the pepsin and pancreatin enzyme components; storage at 2–30 °C is fine for short-term use
- Add enzymes on the day of use; do not store enzyme solutions
Customization Options
- Third fibre-degrading enzyme step
- Enzyme activity matched to your method
- Cat-specific incubation protocol
- Larger kits for routine QC
Ordering Information
BZ706 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research and product testing only. Not for veterinary or human therapeutic use.
