Coagulated Blood Test Soil Kit (Lyophilised Sheep Blood with Protamine) BZ629
Product Code: BZ629
Manufacturer: Biochemazone
Available Pack Sizes: Kits that make 50 mL, 100 mL or 250 mL of soil
Custom Bulk Quantities Available
Product Overview
Biochemazone Coagulated Blood Test Soil Kit BZ629 provides a clotting blood soil for the toughest cleaning tests. Lyophilised (freeze-dried) heparinised sheep blood is reconstituted with purified water and mixed with protamine sulfate just before use; the protamine neutralises the heparin and the blood clots on the test surface, much like dried and clotted blood on surgical instruments.
Protamine-coagulated sheep blood was one of the national test soils in the annexes of ISO/TS 15883-5:2005 (now withdrawn). It is still used to challenge washer-disinfectors, lumened instruments and robotic instruments. The blood has a pH of about 7.4 (range 7.2–7.6).
The kit is supplied dry in two parts: lyophilised heparinised sheep blood and protamine sulfate powder, with reconstitution and mixing instructions. Because the soil starts to clot once mixed, it must be made fresh. The dry kit is of animal origin, ships at room temperature and has a 2-year shelf life unopened. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Clotting Blood Soil – Coagulates on the test surface
✅ Two-Part Kit – Lyophilised sheep blood plus protamine sulfate powder
✅ pH About 7.4 – Range 7.2–7.6
✅ Worst-Case Challenge – Clotted blood is hard to remove
✅ Mix Fresh – Clear reconstitution and mixing instructions included
✅ Consistent Batch-to-Batch – Repeatable cleaning challenge
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧼 Washer-Disinfector Testing
• Cleaning-efficacy type testing
• Load and cycle challenge studies
🤖 Complex Instruments
• Lumened and robotic instrument cleaning
• Endoscope accessory cleaning research
🔬 Detergent Development
• Enzymatic detergent efficacy on clotted blood
• Pre-soak and pre-clean product testing
Usage Areas
- Washer-disinfector manufacturers
- Surgical and robotic instrument makers
- Cleaning-validation laboratories
- Detergent manufacturers
- Hospital sterile-services departments
Typical Properties
- Appearance: Dark red blood (part A); clear protamine solution (part B)
- pH: about 7.4 (range 7.2–7.6)
- Composition: Lyophilised heparinised defibrinated sheep blood; protamine sulfate (dry powder)
- Biological Status: Contains animal blood; handle with standard laboratory precautions
Storage & Stability
- Storage Temperature: 2–30 °C (dry kit)
- Shelf Life: 2 years unopened (dry powder)
- Shipping: Room temperature
- Best kept at 2–8 °C for prolonged storage and stability of the blood-protein components; storage at 2–30 °C is fine for short-term use
- Reconstitute and mix parts A and B immediately before use; use reconstituted blood the same day
Customization Options
- Other animal bloods
- Larger kits for production validation
- Standing orders for routine testing
Ordering Information
BZ629 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ Contains animal blood. For laboratory testing only. Not for human or animal use.
