Simulated Body Fluid (SBF) Dry Kit – ISO 23317 Kokubo SBF, pH 7.40 BZ585

Simulated Body Fluid (SBF) Dry Kit to ISO 23317 (Kokubo SBF) BZ585

Product Code: BZ585
Manufacturer: Biochemazone
Available Pack Sizes: Kits that make 500 mL or 1 L; 5-kit multipack
Custom Bulk Quantities Available

Product Overview

Biochemazone Simulated Body Fluid (SBF) Dry Kit BZ585 is a pre-weighed dry powder, supplied in one bottle, for preparing Kokubo simulated body fluid as described in ISO 23317 (Implants for surgery – Materials – Simulated body fluid (SBF) preparation procedure and test method to detect apatite formation in SBF). SBF has ion concentrations nearly equal to human blood plasma and is used to screen the apatite-forming (bioactive) ability of bone-contacting materials.

SBF is supersaturated with respect to apatite, so it is best prepared fresh and used soon after. Each kit supplies the Kokubo SBF reagents – sodium chloride, sodium hydrogen carbonate, potassium chloride, dipotassium hydrogen phosphate trihydrate, magnesium chloride hexahydrate, calcium chloride, sodium sulfate and tris(hydroxymethyl)aminomethane – pre-weighed and blended as a dry powder in a single bottle. The user simply mixes the powder with deionized water, following the Instructions for Use provided with the kit, to obtain SBF at pH 7.40 at 36.5 °C.

The dry kit removes weighing errors, ships without refrigeration and stores for two years, so each experiment starts from freshly made SBF. Kits are made under Biochemazone’s ISO 9001 / GMP quality system. For ready-to-use liquid SBF see BZ173 and our ISO 23317 SBF.

Key Features & Benefits

✅ Made for ISO 23317 – Reagents for Kokubo SBF
✅ Pre-Weighed Powder in One Bottle – Just mix with deionized water as instructed
✅ Freshly Made SBF – Avoids precipitation seen in stored liquid SBF
✅ Target pH 7.40 at 36.5 °C – When prepared as per the Instructions for Use provided
✅ Long Shelf Life – 24 months dry at room temperature
✅ Low Shipping Cost – Non-hazardous, light kit
✅ Instructions Included – Step-by-step preparation guide
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🦴 Bioactivity Testing
• Apatite-forming ability of implant materials (ISO 23317)
• Bioactive glasses, glass-ceramics and calcium phosphates
• Coated titanium and metal implants

🧪 Biomaterial Development
• Biomimetic apatite coating of scaffolds
• Degradation and ion-release studies
• Screening of new bone-graft substitutes

🎓 Research & Teaching
• University biomaterials laboratories
• Student practicals on bioactivity
• Inter-laboratory comparisons

Usage Areas

• Orthopaedic and dental implant manufacturers
• Biomaterials research laboratories
• Bioactive glass and ceramic developers
• Contract testing laboratories
• Universities and teaching laboratories

How to Use

1. Read the Instructions for Use provided with the kit before starting.
2. Empty the full contents of the bottle into a clean plastic beaker and add deionized water in the volume given in the instructions for your kit size (500 mL or 1 L).
3. Mix until the powder has dissolved completely, as described in the instructions.
4. Use promptly; store at 5–10 °C and discard if any precipitate forms.

Typical Properties

• Form: Pre-weighed dry powder in one bottle
• Final pH: 7.40 at 36.5 °C (prepared as per the Instructions for Use)
• Ion concentrations (mM): Na⁺ 142.0, K⁺ 5.0, Mg²⁺ 1.5, Ca²⁺ 2.5, Cl⁻ 147.8, HCO₃⁻ 4.2, HPO₄²⁻ 1.0, SO₄²⁻ 0.5
• Buffer: Tris / hydrochloric acid
• Not included: Deionized water

Storage & Stability

• Storage Temperature: 2–30 °C
• Shelf Life: 2 years unopened (dry powder)
• Shipping: Room temperature
• Prepared SBF: store at 5–10 °C and use within 30 days; discard if cloudy

Customization Options

• Revised (r-SBF), corrected (c-SBF) and newly improved (n-SBF) kits
• 1.5× and concentrated SBF kits
• Private-label and bulk supply

Ordering Information

BZ585 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com

⚠️ For research and laboratory testing only. Not for human or animal use.