5X TBE buffer, Tris-borate-EDTA electrophoresis concentrate, BZ209 – BioChemazone 500 mL bottle

5X TBE Buffer (Tris-Borate-EDTA), pH 8.3 – Electrophoresis Running Buffer (BZ209)

Price range: $15.00 through $39.00

5X TBE Buffer (Tris-Borate-EDTA) Electrophoresis Concentrate BZ209

Product Code: BZ209
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L
Custom Bulk Quantities Available

Product Overview

Biochemazone 5X TBE Buffer BZ209 is a five-times concentrated Tris-borate-EDTA buffer for agarose and polyacrylamide gel electrophoresis of DNA. Diluted 1:10 to the common 0.5X working strength, it is used both to cast the gel and as the tank running buffer.

TBE holds its pH well during long or high-voltage runs and gives sharp, well-resolved bands for small DNA fragments (below about 1 kb). The 5X strength is less prone to precipitation than 10X TBE, so it is convenient to keep on the bench. EDTA chelates divalent ions and protects DNA from nucleases during the run.

Borate inhibits many enzymes, so use TAE buffer (BZ208) when DNA will be recovered from the gel for cloning. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.

Key Features & Benefits

✅ 5X Concentrate – Dilute 1:10 for 0.5X or 1:5 for 1X
✅ Less Precipitation – 5X stays in solution better than 10X TBE
✅ Sharp Small-Fragment Bands – Ideal for DNA below about 1 kb and for PAGE
✅ High Buffering Capacity – Stable pH during long runs
✅ Contains EDTA – Protects nucleic acids from nucleases
✅ Gel & Tank Buffer – Use the same dilution to cast and run
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems

Applications

🧬 Nucleic Acid Electrophoresis
• Agarose gels for PCR products and restriction digests
• Native and denaturing PAGE
• Genotyping and fragment-analysis gels

🔬 Molecular Biology QC
• Plasmid and genomic DNA checks
• Oligonucleotide analysis on PAGE

🎓 Teaching Laboratories
• Student electrophoresis practicals

Usage Areas

  • Molecular biology and genetics laboratories
  • Diagnostic and assay R&D
  • Forensic and genotyping laboratories
  • Biotechnology companies
  • Teaching laboratories

Composition (5X, per litre)

ComponentConcentrationAmount per litre
Tris base445 mM53.9 g
Boric acid445 mM27.5 g
EDTA (disodium salt, dihydrate)10 mM3.72 g
High-purity water (ISO 3696)—to 1 L

0.5X working solution (1:10 dilution): 44.5 mM Tris, 44.5 mM boric acid, 1 mM EDTA, pH about 8.3.

Typical Properties

  • Appearance: Clear, colourless liquid
  • pH: About 8.3 at working strength
  • Concentration: 5X
  • Dilution: 1 + 9 (0.5X) or 1 + 4 (1X) with high-purity water

Available Formulations

  • 5X TBE (this product, BZ209)
  • 10X TBE (BZ202)
  • 50X TAE (BZ208)
  • Custom: 1X or 0.5X ready to use

Storage, Shelf Life & Transportation

  • Storage Temperature: 2–30 °C
  • Shelf Life: 1 year for unopened bottles
  • Transportation: Room temperature (ambient shipping)
  • Version with Preservatives: Available on request
  • Powder Version: Available on request
  • If a precipitate forms, warm to 37 °C and mix until dissolved before diluting
  • Keep tightly closed

Customization Options

  • 10X, 1X or 0.5X ready to use
  • Different EDTA concentration
  • Bulk 4 L, 10 L and 20 L containers

Ordering Information

BZ209 is available in standard laboratory sizes or OEM/bulk supply formats.

📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
Email: info@biochemazone.com
Website: www.biochemazone.com

⚠️ For laboratory research use only. Not for human or animal injection or therapeutic use.

Description

Product overview

5X TBE Buffer (BZ209) is a Tris-borate-EDTA electrophoresis concentrate that dilutes 1:10 to 0.5X running buffer, pH 8.3, for agarose and polyacrylamide gels.

  • Catalogue no.: BZ209
  • Concentration: 5X (445 mM Tris-borate, 10 mM EDTA)
  • pH: About 8.3 at working strength
  • Dilution: 1:10 (0.5X) or 1:5 (1X)
  • Pack sizes: 100 mL, 200 mL, 500 mL, 1 L
  • Storage: 2–30 °C
  • Shelf life: 1 year (unopened bottle)
  • On request: Version with preservatives; powder version
  • Transportation: Room temperature
  • Use: Laboratory research use only

See also: 10X TBE (BZ202) · 50X TAE (BZ208) · 1X TE Buffer pH 8.0 (BZ210)

Frequently asked questions

How do I dilute 5X TBE?

For the common 0.5X running strength, mix 1 part BZ209 with 9 parts high-purity water. For 1X, mix 1 part with 4 parts water. Use the same dilution for the gel and the tank.

Why choose 5X TBE instead of 10X?

5X TBE is less likely to precipitate during storage than 10X TBE, so it is easier to keep ready on the bench. Both give the same working buffer after dilution.

Is TBE suitable for gel extraction?

TBE works well for analysis, but borate can inhibit enzymes used after gel extraction. For DNA recovery and cloning, use TAE (BZ208).

What is the pH of the TBE buffer?

About pH 8.3 at working strength, the standard value for Tris-borate-EDTA running buffer.

How should 5X TBE be stored?

Store at 2–30 °C, tightly closed. Unopened bottles have a 1-year shelf life and ship at room temperature.

5X TBE Buffer

5X TBE Buffer composition and customer’s specified configurations can be delivered upon request. Concentration, EDTA Level, pH Value, Container Size, and Contents of the buffer are customizable.
Contact us for Customization: info@biochemazone.com
Transportation and storage: Transportation at room temperature, storage at 2–30 ℃
We provide shipping facilities worldwide.

quality-assurance
Quality-Control-Approved
iso
iata
dubai-accreditation
iaf

Documents

Certificates of Analysis are issued per production batch. Please add the lot number or your order number to the request (catalogue no. BZ209 is filled in for you).

Additional information

Pack Size

100 mL, 200 mL, 500 mL, 1 L

Important Notes

  • Research Use Only: Biochemazone™ materials are intended strictly for research and development purposes in laboratory and industrial settings.
  • No Refunds or Exchanges: All sales are final. Biochemazone™ does not permit exchanges or offer refunds under any circumstances.
  • Customs & Import Responsibilities: Customs clearance, duties, taxes, and tariffs are the sole responsibility of the consignee/importer. Biochemazone™ is not liable for delays, rejections, or any additional costs incurred during the customs process, and such issues will not justify order cancellations or refunds.
  • Order Cancellations: Once an order is placed, it cannot be cancelled. No refunds will be issued for cancelled or undelivered orders.
  • Indemnification: The buyer agrees to indemnify and hold harmless Biochemazone™, its affiliates, and personnel from any claims, damages, liabilities, or expenses arising from the use of its products or services.

Terms & Conditions: All orders and communications are subject to Biochemazone™’s standard Terms & Conditions.

Disclaimer: Images and colors of the formulation displayed are for portrayal purposes only. Actual color and consistency may vary.

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