Description
Product overview
50X TAE Buffer (BZ208) is a 0.22 µm-filtered Tris-acetate-EDTA concentrate that dilutes 1:50 to 1X running buffer, pH 8.3, for agarose gel electrophoresis and gel extraction.
- Catalogue no.: BZ208
- Concentration: 50X (2 M Tris-acetate, 50 mM EDTA)
- pH: 8.3 at 1X
- Dilution: 1 part + 49 parts water
- Pack sizes: 100 mL, 200 mL, 500 mL, 1 L
- Storage: 2–30 °C
- Shelf life: 1 year (unopened bottle)
- On request: Version with preservatives; powder version
- Transportation: Room temperature
- Use: Laboratory research use only
Frequently asked questions
How do I dilute 50X TAE to 1X?
Add 20 mL of BZ208 to 980 mL of high-purity water (1 part + 49 parts). Use 1X TAE both to cast the agarose gel and to fill the electrophoresis tank.
When should I choose TAE instead of TBE?
Choose TAE for large DNA fragments, genomic DNA and supercoiled plasmids, and whenever DNA will be recovered from the gel for enzymatic steps. Choose TBE (BZ202) for small fragments, PAGE and long high-voltage runs.
Why does TAE need replacing during long runs?
TAE has a lower buffering capacity than TBE. During long or high-voltage runs the buffer becomes exhausted, so replace it or recirculate it between the electrodes.
Is BZ208 filtered?
Yes. The concentrate is filtered through a 0.22 µm membrane.
How should 50X TAE be stored?
Store at 2–30 °C, tightly closed. Unopened bottles have a 1-year shelf life and ship at room temperature.
50X TAE Buffer
50X TAE Buffer composition and customer’s specified configurations can be delivered upon request. Concentration, EDTA Level, pH Value, Container Size, and Contents of the buffer are customizable.
Contact us for Customization: info@biochemazone.com
Transportation and storage: Transportation at room temperature, storage at 2–30 ℃
We provide shipping facilities worldwide.
