McDougall’s Artificial Saliva (Rumen Buffer for In-Vitro Fermentation) BZ668
Product Code: BZ668
Manufacturer: Biochemazone
Available Pack Sizes: 1 L, 5 L, 10 L, 20 L
Custom Bulk Quantities Available
Product Overview
Biochemazone McDougall’s Artificial Saliva BZ668 is the bicarbonate–phosphate buffer that imitates the saliva of sheep and cattle. Described by McDougall (1948), it is the standard buffer for mixing with rumen fluid in in-vitro fermentation, gas-production and digestibility studies of feeds for cattle, sheep and goats.
The buffer contains sodium bicarbonate and disodium phosphate with small amounts of sodium, potassium, calcium and magnesium salts. As supplied it has a pH of about 8.2–8.5; after saturation with carbon dioxide and mixing with rumen fluid, it holds the incubation at about pH 6.8, as saliva does in the living rumen.
Gas the buffer with CO₂ and warm it to 39 °C before use, and add reducing agent, resazurin or trace minerals if your method requires them. Check the recipe and gassing steps in your method. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ McDougall (1948) Buffer – The classic ruminant artificial saliva
✅ Bicarbonate–Phosphate – Buffers fermentation acids
✅ pH About 8.2–8.5 – About 6.8 after CO₂ gassing
✅ Ready-to-Use – No salt weighing on site
✅ Up to 20 L – For large incubation series
✅ No Reducing Agent – Add per your own protocol
✅ ISO 3696 Grade Water – Prepared using high-purity laboratory water
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🐄 Rumen Fermentation
• In-vitro gas-production tests
• Batch and continuous rumen culture
• Methane-mitigation screening
🌾 Feed Evaluation
• In-vitro digestibility of forages
• Feed-additive and enzyme trials
🎓 Research & Teaching
• Animal-nutrition courses
• Rumen microbiology research
Usage Areas
- Feed and forage laboratories
- Animal-nutrition departments
- Feed-additive companies
- Agricultural research institutes
- Universities
Typical Properties
- Appearance: Clear, colourless liquid
- pH: about 8.2–8.5 as supplied; about 6.8 after CO₂ saturation
- Composition: Sodium bicarbonate, disodium phosphate, and sodium, potassium, calcium and magnesium salts
- Water Grade: ISO 3696 purified water
- Biological Status: Contains no rumen fluid or animal material
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Keep tightly closed to limit CO₂ loss; gas before use
Customization Options
- Dry salt packs
- Menke and Steingass buffer
- Buffer with reducing agent and resazurin
- Carboys for continuous-culture systems
Ordering Information
BZ668 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research and product testing only. Not for veterinary or human therapeutic use.
