Interference Test Set – Haemolysed, Icteric and Lipaemic Serum Simulants BZ590
Product Code: BZ590
Manufacturer: Biochemazone
Available Pack Sizes: 100 mL, 200 mL, 500 mL, 1 L per interferent, or sets of four
Custom Bulk Quantities Available
Product Overview
Biochemazone Interference Test Set BZ590 provides serum simulants spiked with the three endogenous interferents that most often disturb clinical-chemistry and immunoassay results: haemoglobin (haemolysis), bilirubin (icterus) and lipid (lipaemia). Interference testing of this kind is described in the CLSI guideline EP07 (Interference Testing in Clinical Chemistry).
Each interferent is supplied at a high stock level in the same protein-containing serum simulant, together with an unspiked blank in the set. Mixing the stock and blank in different ratios gives a dose series, so the concentration at which an assay begins to be affected can be found. Stock levels are about 10 g/L haemoglobin, 40 mg/dL (684 µmol/L) bilirubin and 20 g/L lipid emulsion; the measured values are stated on the certificate. The base is buffered to pH 7.4 (range 7.3–7.5).
The haemoglobin is of bovine origin and the lipid is a soybean-oil emulsion. Each batch is prepared with ISO 3696 compliant high-purity water under Biochemazone’s ISO 9001 / GMP quality system.
Key Features & Benefits
✅ Three Endogenous Interferents – Haemoglobin, bilirubin and lipid
✅ Matched Blank – Same serum simulant without interferent, for dose series
✅ High Stock Levels – About 10 g/L haemoglobin, 40 mg/dL bilirubin, 20 g/L lipid
✅ Certified Values – Measured concentrations on each certificate
✅ pH 7.4 (Range 7.3–7.5) – Physiological serum pH
✅ Protein-Containing Base – Behaves like serum in immunoassays and chemistry tests
✅ Single Interferents or Sets – Buy what your study needs
✅ Made in Canada – Manufactured under ISO 9001 / GMP quality systems
Applications
🧪 Assay Development
• Haemolysis, icterus and lipaemia interference studies
• Dose–response series to find interference thresholds
• Serum-index (HIL) calibration checks
🩺 IVD Verification
• Interference data for technical files and submissions
• Point-of-care and rapid-test robustness testing
• Method comparisons between analysers
🔬 Research & Teaching
• Pre-analytical error studies
• Laboratory-medicine teaching
Usage Areas
- IVD and immunoassay developers
- Clinical-chemistry analyser manufacturers
- Point-of-care and rapid-test companies
- Clinical laboratories verifying methods
- Universities and laboratory-medicine programmes
Typical Properties
- Appearance: Red (haemolysed), yellow-orange (icteric), milky (lipaemic), pale yellow (blank)
- pH: 7.4 (range 7.3–7.5)
- Haemolysed stock: about 10 g/L bovine haemoglobin
- Icteric stock: about 40 mg/dL (684 µmol/L) bilirubin
- Lipaemic stock: about 20 g/L soybean-oil lipid emulsion
- Base: Buffered serum simulant with albumin
- Water Grade: ISO 3696 purified water
Storage & Stability
- Storage Temperature: 2–30 °C
- Shelf Life: 1 year for unopened bottles
- Shipping: Room temperature
- Best kept at 2–8 °C for prolonged storage and stability of the protein (albumin and haemoglobin) components; storage at 2–30 °C is fine for short-term use
- Protect the icteric stock from light (bilirubin degrades in light); mix the lipaemic stock gently before use
Customization Options
- Other stock levels or ready-made dilution series
- Additional interferents (for example biotin, ascorbic acid, rheumatoid factor substitutes)
- Plasma-based or protein-free matrices
- Custom pack sizes and bulk supply
Ordering Information
BZ590 is available in standard laboratory sizes or OEM/bulk supply formats.
📄 MSDS, Technical Datasheet, and Certificate of Analysis (CoA) available upon request
📧 Email: info@biochemazone.com
🌐 Website: www.biochemazone.com
⚠️ For research and assay development only. Not for clinical diagnosis. Not for human or animal use.
