Culture ACSF (Artificial Cerebrospinal Fluid)BZ460
This simulated fluid is only used in the field of scientific research and For in vitro use only.
Pack Size: 100 mL, 200 mL, 500 mL, 1000 mL
pH is 7.4 (can be customized on request as per the customer’s project requirement)
Validity: 6 months
Transportation and storage: transportation at room temperature, storage at 2-8 ℃.
Culture ACSF Features & Notes
- Description:
Culture Artificial cerebrospinal fluid is a laboratory-designed fluid that replicates the ionic composition and properties of natural cerebrospinal fluid. It contains water, essential electrolytes (such as sodium, potassium, calcium, magnesium, and chloride), glucose, and other key components to maintain the viability of neural tissues and cells. This formulation is commonly used in research areas such as neurophysiology, brain slice electrophysiology, and neural cell culture, providing an environment conducive to studying brain function and neural interactions.
- Storage and Transportation:
Store Culture ACSF at 4°C to preserve its stability and prevent changes in pH or degradation of components. The solution can be transported at room temperature for short periods without affecting its composition or buffering capacity.
- Usage Restrictions:
Culture ACSF is intended strictly for research purposes and should be handled by trained professionals within a controlled laboratory setting. It is not approved for clinical diagnostics, direct application on humans, or pharmaceutical use. The fluid is specifically designed for experiments related to neural tissue culture, electrophysiology, and drug testing.
- Safety Precautions:
When handling Culture ACSF, standard laboratory safety protocols should be followed, including the use of lab coats, disposable gloves, and eye protection. Avoid direct contact with the eyes or skin. In case of accidental exposure, flush the affected area with water. Dispose of the solution and any contaminated materials according to your institution’s laboratory waste guidelines to prevent contamination and ensure proper environmental safety.
Culture ACSF is used primarily by research laboratories to study the properties and behaviors of cerebrospinal fluid, including its interactions with neural tissues, brain cells, and various physiological processes. It is widely employed in neurophysiology experiments, electrophysiological recordings, and drug testing to explore neuronal functions and interactions in controlled environments.
- Viscosity:
The viscosity of Culture ACSF is carefully controlled to match the low viscosity of the natural human cerebrospinal fluid, ensuring it flows freely through neural tissues. This water-like consistency allows for accurate simulation of fluid dynamics in the brain, particularly in experiments involving neural tissue viability, electrophysiological studies, and fluid transport across neural interfaces.
- Density:
The density of Culture ACSF is calibrated to reflect that of natural CSF, providing accurate and reproducible results in experiments studying neural tissue interactions, fluid circulation, and how different compounds, drugs, or materials interact with the fluid in vitro. This consistency ensures reliable results in experiments simulating CSF flow and absorption within the central nervous system.
- pH Level:
The pH of Culture ACSF is typically set between 7.35 and 7.45, reflecting the neutral-to-slightly-alkaline nature of the natural cerebrospinal fluid. This pH range is essential for maintaining the physiological conditions needed to support neural tissue health, ensuring neurons and glial cells remain viable and functional throughout experiments.
- Stability:
Culture ACSF is designed to remain chemically and physically stable over the duration of testing, with no significant fluctuations in ionic composition, pH, or other properties. This stability is crucial for producing reliable, reproducible data in long-term neural studies, such as brain slice electrophysiology, neural tissue culture, and drug interaction assays.
- Color:
Culture ACSF is usually clear and colorless, resembling the natural appearance of cerebrospinal fluid. This visual clarity is important in experiments involving optical measurements, neural imaging, or electrophysiological monitoring, where transparency of the fluid helps ensure clear observations of brain tissue and neural activity without interference.
Culture ACSF Applications:
- Testing and Calibration:
Culture ACSF is used for the testing and calibration of medical and research devices that interact with cerebrospinal fluid. These include devices for neurophysiological measurements, such as electrodes for brain slice electrophysiology, neural sensors, and drug delivery systems that rely on accurate simulation of CSF properties. Its use ensures that such devices function properly in fluid environments that closely resemble real cerebrospinal conditions.
- Research and Development:
Culture ACSF plays a crucial role in research and development in neuroscience, neuropharmacology, and brain health. It supports studies focused on neural tissue interactions, fluid transport in the brain, and the effects of drugs on the central nervous system. Researchers use ACSF to investigate conditions like neurodegenerative diseases, brain injuries, and the behavior of neurons and glial cells in vitro. Additionally, it aids in developing therapies and treatments for brain-related disorders, as well as the creation of implantable devices that interact with cerebrospinal fluid.
- Training and Education:
Culture ACSF is employed in educational and training programs for students and professionals in fields such as neuroscience, neurology, and medical device engineering. It provides a realistic medium for hands-on learning about cerebrospinal fluid composition, neural tissue health, and how neural interactions can be studied in vitro. It also allows students and trainees to simulate and understand CSF behavior and its importance in physiological and pathological processes without the need for live biological samples.
This ready-to-use solution has the same composition as Culture ACSF and closely resembles Real Culture CSF. This formulation may contains non-toxic preservatives & Antibiotics (optional) to avoid Mold & bacteria growth and can be stored at room temperature. The pH of the Synthetic Cerebrospinal fluid Formulation Stabilized is 7.4 (customizable on request as per the customer’s project requirements).
We also accept customization in formulation, minerals, organic content, pack size, preservatives, and pH. The powdered Culture ACSF is also available for this product. Please send us an email for more information.
Biochemazone™ offers a diverse selection of Artificial fluids for the testing & Diagnostic of various diseases in Vitro laboratory and Research. Check out our various state biofluid inventory to see what’s ready to ship today.
For a custom request, please send us an email at info@biochemazone.com
Packaging: 100 mL to 1000 mL (Also available in 5, 10, and 25-Liter Containers at economical Bulk Discounted Prices, kindly send us an email for bulk purchases on discounts for your Industrial and academic requirements)
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