Citric Acid-Sodium Citrate Buffer (0.1mol/L, pH 4.5) – Sterile Solution for Laboratory Use
The Citric Acid-Sodium Citrate Buffer (0.1mol/L, pH 4.5) Sterile is a high-quality laboratory reagent widely used in biochemical and pharmaceutical applications. This buffer solution plays a crucial role in immunohistochemistry, protein analysis, and various research experiments requiring precise pH control. Manufactured under stringent quality standards, it ensures consistency, reliability, and safety in laboratory procedures.
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What is Citric Acid-Sodium Citrate Buffer Solution?
Citric acid-sodium citrate buffer is a pH-regulated solution prepared by mixing citric acid and sodium citrate in specific ratios. The buffer maintains a stable acidic pH of 4.5, making it an essential reagent in scientific research, particularly in protein studies, immunohistochemistry, and biochemical assays.
Key Specifications
- Concentration: 0.1 mol/L (customizable)
- pH Value: 4.5 (customizable)
- Pack Sizes: 100 mL, 200 mL, 500 mL, 1000 mL (customizable)
- Storage Conditions: Store at room temperature (RT)
- Shelf Life: 12 months
- Sterilization Method: 0.2 μm filter sterilization
Applications of Citric Acid-Sodium Citrate Buffer
1. Immunohistochemistry and Tissue Processing
This buffer is widely used in immunohistochemistry (IHC) to expose antigen epitopes by breaking protein cross-links caused by aldehyde fixatives. It significantly improves the staining intensity and clarity of tissue samples, making it an essential reagent in pathology and diagnostic research.
2. Protein and Enzyme Stabilization
Maintaining a controlled pH is crucial for protein stability. The citric acid-sodium citrate buffer helps preserve enzymatic activity and prevent protein degradation during experiments.
3. Biochemical and Molecular Biology Assays
Many biochemical assays, including enzyme-linked immunosorbent assays (ELISA) and DNA/RNA electrophoresis, require a precisely controlled pH environment. This buffer ensures optimal conditions for molecular interactions.
4. Pharmaceutical and Medical Research
Sodium citrate is commonly used as an anticoagulant in blood sample collection. Additionally, the citric acid-sodium citrate buffer is employed in drug formulation and testing processes.
Advantages of Using Citric Acid-Sodium Citrate Buffer (0.1mol/L, pH 4.5)
1. Reliable pH Maintenance
With its precise formulation, this buffer maintains a consistent pH of 4.5, ensuring experimental accuracy and reproducibility.
2. Sterile and Ready to Use
The solution is sterile, making it ideal for sensitive biological and clinical applications. It eliminates the need for additional filtration or preparation.
3. Customizable Concentration and Volume
Users can select different concentrations and volumes based on specific research needs, making it a versatile choice for laboratories.
4. High Stability and Long Shelf Life
Stored at room temperature, the buffer remains stable for 12 months, reducing wastage and frequent re-preparations.
How to Use Citric Acid-Sodium Citrate Buffer in the Laboratory
The application method depends on the experiment. Below is a general guideline:
- Ensure Proper Handling: Wear a lab coat, disposable gloves, and protective eyewear.
- Use As Per Experimental Requirements: Pipette the required volume of buffer into the experimental setup.
- Store Properly After Use: Keep the container tightly closed to prevent pH fluctuations.
Precautions When Handling Citric Acid-Sodium Citrate Buffer
- For laboratory use only: Not intended for pharmaceutical, household, or medical use.
- Avoid contamination: Always use sterilized pipettes and containers to maintain buffer integrity.
- Storage conditions: Keep at room temperature and away from direct sunlight to preserve efficacy.
Why Choose Our Citric Acid-Sodium Citrate Buffer?
1. High-Quality Manufacturing Standards
Our BZ203 buffer is manufactured under strict quality control guidelines, ensuring high purity and consistency in every batch.
2. Versatile Research Applications
This buffer is extensively used across biochemistry, molecular biology, and pharmaceutical research, making it a valuable addition to any laboratory.
3. Customizable Options
We provide customization for concentration, pH levels, and packaging sizes, catering to the unique needs of various scientific research fields.
4. Easy Procurement and Reliable Supply
You can conveniently purchase the Citric Acid-Sodium Citrate Buffer (BZ203) online, with guaranteed quality assurance and timely delivery.
👉 Order Now: Citric Acid-Sodium Citrate Buffer (BZ203)
Frequently Asked Questions (FAQs)
1. What is the purpose of using citric acid-sodium citrate buffer in laboratories?
This buffer is used for maintaining a stable pH in biochemical reactions, immunohistochemistry, enzyme stabilization, and protein assays.
2. Can I customize the pH and concentration of the buffer?
Yes, the buffer is available in customizable pH and concentration options to meet specific research requirements.
3. How should I store citric acid-sodium citrate buffer?
Store the buffer at room temperature (RT) in a tightly sealed container to maintain stability for up to 12 months.
4. Is this buffer sterile?
Yes, the solution is sterilized using a 0.2 μm filter, making it suitable for sensitive laboratory applications.
5. Can this buffer be used for medical or household purposes?
No, this product is strictly for laboratory use only and should not be used for medical, drug, or household applications.
6. How does citric acid-sodium citrate buffer improve immunostaining in histological samples?
It helps to break aldehyde-induced protein cross-links, exposing epitopes in paraffin-embedded tissues and enhancing immunostaining results.
The Citric Acid-Sodium Citrate Buffer (0.1mol/L, pH 4.5) Sterile is an essential reagent for scientific research, ensuring precise pH control and high reliability across various laboratory applications. Whether you’re working in immunohistochemistry, protein stabilization, or molecular biology, this buffer provides an optimal solution.
Order today and enhance your research with a premium-quality buffer:
🔗 Citric Acid-Sodium Citrate Buffer (BZ203)
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