TE Buffer (Tris-EDTA Buffer, pH 8.0): A Comprehensive Guide
Introduction to TE Buffer (Tris-EDTA Buffer, pH 8.0)
Tris-EDTA (TE) buffer, also known as T10E1 buffer, is an essential reagent in molecular biology. This ready-to-use solution has become a cornerstone in procedures involving DNA and RNA protection and storage. With its unique formulation combining Tris (a pH buffer) and EDTA (a chelating agent for divalent ions), TE buffer ensures optimal conditions to protect nucleic acids from degradation.
Available in customizable pH levels and volumes ranging from 100 mL to 1000 mL, TE buffer has versatile applications, including DNA purification, RNA handling, and serving as a washing buffer.
What is TE Buffer?
TE buffer is a buffer solution composed of:
- Tris: A widely used buffering agent, effective in maintaining pH stability within the range of 7.5 to 8.5.
- EDTA: A molecule that binds metal ions like Mg²⁺, which are essential cofactors for nucleases, thereby inactivating these enzymes and protecting DNA or RNA from degradation.
Key Properties of TE Buffer
- Composition:
- Tris-HCl: 10 mM
- EDTA·Na₂: 1 mM
- pH: 8.0
- Formulation:
- Ready-to-use 1× solution, free from DNase enzymes.
- Storage Guidelines:
- Short-term: Store at 4°C for weeks.
- Medium-term: Store at -80°C for months.
- Long-term: Store at -80°C as a precipitate in ethanol.
- Decades: Store dried or at -164°C.
- Packaging Sizes:
- Customizable volumes: 100 mL, 200 mL, 500 mL, and 1000 mL.
Why Use TE Buffer in Molecular Biology?
1. Protects DNA and RNA
TE buffer’s combination of Tris and EDTA ensures nucleic acid stability. Tris maintains a stable pH, while EDTA chelates metal ions, preventing nuclease activity.
2. Ideal for DNA Purification and Storage
TE buffer is widely used in DNA extraction protocols, providing an ideal environment for long-term storage of DNA and RNA samples.
3. Versatile Applications
Beyond DNA and RNA stabilization, TE buffer serves as a washing buffer in various laboratory procedures.
Applications of Tris-EDTA Buffer
1. DNA and RNA Solubilization
TE buffer is used to dissolve DNA and RNA, maintaining their integrity during experiments and storage.
2. DNA Purification
Its nuclease-inhibiting properties make it a preferred choice for purifying DNA from contaminants.
3. Protein Research
Although primarily used for nucleic acids, TE buffer can also assist in protein stabilization during research.
4. Washing Buffer
TE buffer’s gentle nature makes it suitable for use as a washing buffer in various molecular biology protocols.
How to Use TE Buffer Effectively
Preparation Guidelines
TE buffer is supplied as a 1× ready-to-use solution, making it highly convenient. Here are some tips for effective usage:
- Always use sterile techniques to avoid contamination.
- Autoclave the buffer if sterility is required.
- Customize the pH if necessary, depending on the application.
Safety Precautions
- Wear lab coats and disposable gloves while handling TE buffer.
- Avoid contact with skin and eyes.
Storage Instructions for TE Buffer
To ensure the buffer remains effective, follow these storage guidelines:
- Store at room temperature for a maximum of 12 months.
- For extended storage, keep at 4°C or -80°C.
How to Prepare 10X Tris-EDTA Buffer
If you need a concentrated TE buffer, follow this simple recipe for a 10X solution:
| Component | Amount | Concentration |
|---|---|---|
| Tris-Cl (pH 8.0) | 15.759 g | 0.1 M |
| EDTA (pH 8.0) | 2.92 g | 0.01 M |
| Distilled Water | Adjust to 1 L | — |
Steps
- Measure 800 mL of distilled water and pour it into a container.
- Add 15.759 g of Tris-Cl to the water.
- Add 2.92 g of EDTA.
- Add distilled water until the volume reaches 1 L.
This 10X solution can be diluted to 1X for routine use.
Advantages of Tris-EDTA Buffer
- Ready-to-Use: Eliminates the need for time-consuming preparation.
- Stable pH: Maintains optimal conditions for nucleic acid stability.
- Versatile: Suitable for DNA/RNA storage, purification, and washing.
- Customizable: Tailor pH and concentration to meet specific experimental needs.
Frequently Asked Questions (FAQs)
1. What is the purpose of TE buffer in DNA storage?
TE buffer stabilizes and protects DNA by maintaining a stable pH and inhibiting nuclease activity through EDTA’s metal ion chelation.
2. Can TE buffer be used for RNA storage?
Yes, TE buffer is equally effective for storing RNA, helping to protect it from degradation.
3. How should I store TE buffer for long-term use?
For long-term storage, TE buffer can be kept at -80°C as an ethanol precipitate or stored dried.
4. Is TE buffer customizable?
Yes, this product allows customization of pH and concentration to suit various applications.
5. Can TE buffer be used as a washing buffer?
Absolutely! TE buffer’s gentle composition makes it ideal for washing nucleic acids and other biomolecules.
6. Does TE buffer degrade over time?
When stored properly at recommended temperatures, TE buffer remains stable for up to 12 months or longer.
Where to Buy TE Buffer (Tris-EDTA Buffer, pH 8.0)
Looking for high-quality TE buffer for your lab? Purchase the 1×TE Buffer, pH 8.0 (BZ210) today at Biochemazone™. Available in customizable volumes and concentrations, this ready-to-use buffer ensures reliable performance in all your molecular biology experiments.
Conclusion
TE buffer (Tris-EDTA buffer, pH 8.0) is an indispensable reagent in molecular biology, offering unmatched stability and protection for DNA and RNA. With adjustable pH and various packaging options, it is a versatile tool for researchers. Ensure optimal performance in your experiments by choosing a high-quality TE buffer that meets your laboratory needs.
